Evans T J, Buttery L D, Carpenter A, Springall D R, Polak J M, Cohen J
Department of Infections Diseases and Bacteriology, Royal Postgraduate Medical School, London, United Kingdom.
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9553-8. doi: 10.1073/pnas.93.18.9553.
Although the production of NO within rodent phagocytes is well-characterized, its production and function within human phagocytes are less clear. We show here that neutrophils within human buffy coat preparations stimulated with a mixture of interleukin 1, tumor necrosis factor alpha, and interferon gamma contain inducible NO synthase mRNA and protein, one of the enzymes responsible for NO production. The protein colocalizes with myeloperoxidase within neutrophil primary granules. Using an inhibitor of NO synthase, L-N-monomethyl arginine, we show that activity of this enzyme is required for the formation of nitrotyrosine around phagocytosed bacteria, most likely through the intermediate production of peroxynitrite, a reaction product of NO and superoxide anions.
虽然啮齿动物吞噬细胞内一氧化氮（NO）的产生已得到充分表征，但其在人类吞噬细胞内的产生及功能仍不太清楚。我们在此表明，用人白细胞介素1、肿瘤坏死因子α和干扰素γ混合物刺激的人类血沉棕黄层制剂中的中性粒细胞含有诱导型一氧化氮合酶mRNA和蛋白质，这是负责产生NO的酶之一。该蛋白质与中性粒细胞初级颗粒内的髓过氧化物酶共定位。使用一氧化氮合酶抑制剂L-N-单甲基精氨酸，我们表明该酶的活性是吞噬细菌周围硝基酪氨酸形成所必需的，最有可能是通过过氧亚硝酸盐的中间产生，过氧亚硝酸盐是NO和超氧阴离子的反应产物。
