• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

SP-A基因靶向小鼠中表面活性剂功能和结构的改变

Altered surfactant function and structure in SP-A gene targeted mice.

作者信息

Korfhagen T R, Bruno M D, Ross G F, Huelsman K M, Ikegami M, Jobe A H, Wert S E, Stripp B R, Morris R E, Glasser S W, Bachurski C J, Iwamoto H S, Whitsett J A

机构信息

Children's Hospital Medical Center, Department of Pediatrics, Cincinnati, OH 45229-3039, USA.

出版信息

Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9594-9. doi: 10.1073/pnas.93.18.9594.

DOI:10.1073/pnas.93.18.9594
PMID:8790375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC38473/
Abstract

The surfactant protein A (SP-A) gene was disrupted by homologous recombination in embryonic stem cells that were used to generate homozygous SP-A-deficient mice. SP-A mRNA and protein were not detectable in the lungs of SP-A(-/-) mice, and perinatal survival of SP-A(-/-) mice was not altered compared with wild-type mice. Lung morphology, surfactant proteins B-D, lung tissue, alveolar phospholipid pool sizes and composition, and lung compliance in SP-A(-/-) mice were unaltered. At the highest concentration tested, surfactant from SP-A(-/-) mice produced the same surface tension as (+/+) mice. At lower concentrations, minimum surface tensions were higher for SP-A(-/-) mice. At the ultrastructural level, type II cell morphology was the same in SP-A(+/+) and (-/-) mice. While alveolar phospholipid pool sizes were unperturbed, tubular myelin figures were decreased in the lungs of SP-A(-/-) mice. A null mutation of the murine SP-A gene interferes with the formation of tubular myelin without detectably altering postnatal survival or pulmonary function.

摘要

表面活性蛋白A（SP-A）基因在胚胎干细胞中通过同源重组被破坏，这些胚胎干细胞被用于培育纯合SP-A缺陷小鼠。在SP-A（-/-）小鼠的肺中检测不到SP-A mRNA和蛋白，并且与野生型小鼠相比，SP-A（-/-）小鼠围产期存活率没有改变。SP-A（-/-）小鼠的肺形态、表面活性蛋白B-D、肺组织、肺泡磷脂池大小和组成以及肺顺应性均未改变。在测试的最高浓度下，来自SP-A（-/-）小鼠的表面活性剂产生的表面张力与（+/+）小鼠相同。在较低浓度下，SP-A（-/-）小鼠的最小表面张力更高。在超微结构水平上，SP-A（+/+）和（-/-）小鼠的II型细胞形态相同。虽然肺泡磷脂池大小未受干扰，但SP-A（-/-）小鼠肺中的管状髓磷脂结构减少。小鼠SP-A基因的无效突变会干扰管状髓磷脂的形成，而不会明显改变出生后的存活率或肺功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/5004139d9ec0/pnas01522-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/68bc53812672/pnas01522-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/c568c253cdfb/pnas01522-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/eaebe40d9655/pnas01522-0307-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/2bee6d108bce/pnas01522-0307-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/5004139d9ec0/pnas01522-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/68bc53812672/pnas01522-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/c568c253cdfb/pnas01522-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/eaebe40d9655/pnas01522-0307-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/2bee6d108bce/pnas01522-0307-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cf5/38473/5004139d9ec0/pnas01522-0309-a.jpg

相似文献

1
Altered surfactant function and structure in SP-A gene targeted mice.SP-A基因靶向小鼠中表面活性剂功能和结构的改变
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9594-9. doi: 10.1073/pnas.93.18.9594.
2
Surfactant protein-D regulates surfactant phospholipid homeostasis in vivo.表面活性蛋白-D在体内调节表面活性物质磷脂稳态。
J Biol Chem. 1998 Oct 23;273(43):28438-43. doi: 10.1074/jbc.273.43.28438.
3
Targeted disruption of the surfactant protein B gene disrupts surfactant homeostasis, causing respiratory failure in newborn mice.表面活性蛋白B基因的靶向破坏会扰乱表面活性剂的稳态，导致新生小鼠呼吸衰竭。
Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7794-8. doi: 10.1073/pnas.92.17.7794.
4
The collagen-like region of surfactant protein A (SP-A) is required for correction of surfactant structural and functional defects in the SP-A null mouse.表面活性蛋白A（SP-A）的胶原样区域是纠正SP-A基因敲除小鼠表面活性剂结构和功能缺陷所必需的。
J Biol Chem. 2001 Oct 19;276(42):38542-8. doi: 10.1074/jbc.M102054200. Epub 2001 Aug 14.
5
GM-CSF mediates alveolar macrophage proliferation and type II cell hypertrophy in SP-D gene-targeted mice.粒细胞-巨噬细胞集落刺激因子介导SP-D基因靶向小鼠的肺泡巨噬细胞增殖和II型细胞肥大。
Am J Physiol Lung Cell Mol Physiol. 2001 Jun;280(6):L1148-56. doi: 10.1152/ajplung.2001.280.6.L1148.
6
Altered stability of pulmonary surfactant in SP-C-deficient mice.SP-C 基因缺陷小鼠中肺表面活性物质稳定性的改变
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6366-71. doi: 10.1073/pnas.101500298. Epub 2001 May 8.
7
Normal surfactant pool sizes and inhibition-resistant surfactant from mice that overexpress surfactant protein A.来自过表达表面活性物质蛋白A的小鼠的正常表面活性物质池大小和抗抑制表面活性物质
Am J Respir Cell Mol Biol. 1999 Sep;21(3):380-7. doi: 10.1165/ajrcmb.21.3.3676.
8
Altered surfactant homeostasis and alveolar type II cell morphology in mice lacking surfactant protein D.缺乏表面活性蛋白D的小鼠中表面活性物质稳态及II型肺泡上皮细胞形态的改变
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11869-74. doi: 10.1073/pnas.95.20.11869.
9
Localization and developmental expression of surfactant proteins D and A in the respiratory tract of the mouse.表面活性蛋白D和A在小鼠呼吸道中的定位及发育表达
Pediatr Res. 1996 Jun;39(6):930-7. doi: 10.1203/00006450-199606000-00002.
10
Surfactant protein A (SP-A) gene targeted mice.
Biochim Biophys Acta. 1998 Nov 19;1408(2-3):296-302. doi: 10.1016/s0925-4439(98)00075-1.

引用本文的文献

1
Cooperation of immune regulators Tollip and surfactant protein A inhibits influenza A virus infection in mice.免疫调节因子Tollip与表面活性蛋白A协同作用可抑制甲型流感病毒感染小鼠。
Respir Res. 2024 May 3;25(1):193. doi: 10.1186/s12931-024-02820-3.
2
Is human labor at term an inflammatory condition?†.足月分娩的人类劳动是否是一种炎症状态？
Biol Reprod. 2023 Jan 14;108(1):23-40. doi: 10.1093/biolre/ioac182.
3
Small Peptide Derivatives Within the Carbohydrate Recognition Domain of SP-A2 Modulate Asthma Outcomes in Mouse Models and Human Cells.

本文引用的文献

1
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
2
Surfactant metabolism in transgenic mice after granulocyte macrophage-colony stimulating factor ablation.
Am J Physiol. 1996 Apr;270(4 Pt 1):L650-8. doi: 10.1152/ajplung.1996.270.4.L650.
3
Alveolar lining layer is thin and continuous: low-temperature scanning electron microscopy of rat lung.肺泡衬里层薄且连续：大鼠肺的低温扫描电子显微镜观察
J Appl Physiol (1985). 1995 Nov;79(5):1615-28. doi: 10.1152/jappl.1995.79.5.1615.
糖识别结构域内的小肽衍生物调节 SP-A2 在小鼠模型和人细胞中的哮喘结局。
Front Immunol. 2022 Jul 8;13:900022. doi: 10.3389/fimmu.2022.900022. eCollection 2022.
4
Comparison of the Toponomes of Alveolar Macrophages From Wild Type and Surfactant Protein A Knockout Mice and Their Response to Infection.肺泡巨噬细胞地名比较来自野生型和表面活性蛋白 A 敲除小鼠及其对感染的反应。
Front Immunol. 2022 Apr 27;13:853611. doi: 10.3389/fimmu.2022.853611. eCollection 2022.
5
A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors ().高原鼢鼠肺表面活性物质中的一种新型同源四聚体血红蛋白（ ） 。 （注：原文括号内内容不完整，翻译时保留原样）
Front Genet. 2022 Mar 15;13:824049. doi: 10.3389/fgene.2022.824049. eCollection 2022.
6
Surfactant Protein-A Function: Knowledge Gained From SP-A Knockout Mice.表面活性蛋白A的功能：从表面活性蛋白A基因敲除小鼠获得的知识
Front Pediatr. 2022 Jan 7;9:799693. doi: 10.3389/fped.2021.799693. eCollection 2021.
7
Single Nucleotide Polymorphisms Interactions of the Surfactant Protein Genes Associated With Respiratory Distress Syndrome Susceptibility in Preterm Infants.与早产儿呼吸窘迫综合征易感性相关的表面活性蛋白基因的单核苷酸多态性相互作用
Front Pediatr. 2021 Oct 4;9:682160. doi: 10.3389/fped.2021.682160. eCollection 2021.
8
CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung.CRISPRi 介导的肺中 NKX2-1 结合位点的功能分析。
Commun Biol. 2021 May 12;4(1):568. doi: 10.1038/s42003-021-02083-4.
9
Surfactant protein A reduces TLR4 and inflammatory cytokine mRNA levels in neonatal mouse ileum.表面活性蛋白 A 降低新生鼠回肠 TLR4 和炎症细胞因子 mRNA 水平。
Sci Rep. 2021 Jan 28;11(1):2593. doi: 10.1038/s41598-021-82219-y.
10
Surfactant Protein A, a Novel Regulator for Smooth Muscle Phenotypic Modulation and Vascular Remodeling-Brief Report.表面活性蛋白 A，一种平滑肌表型调节和血管重构的新型调节因子——简短报告。
Arterioscler Thromb Vasc Biol. 2021 Feb;41(2):808-814. doi: 10.1161/ATVBAHA.120.314622. Epub 2020 Dec 3.
4
Involvement of granulocyte-macrophage colony-stimulating factor in pulmonary homeostasis.粒细胞-巨噬细胞集落刺激因子在肺稳态中的作用。
Science. 1994 Apr 29;264(5159):713-6. doi: 10.1126/science.8171324.
5
Temporal-spatial distribution of SP-B and SP-C proteins and mRNAs in developing respiratory epithelium of human lung.人肺发育中呼吸上皮内表面活性蛋白B和表面活性蛋白C的时空分布及mRNA表达
J Histochem Cytochem. 1994 Sep;42(9):1187-99. doi: 10.1177/42.9.8064126.
6
Binding of surfactant protein A to the lipid A moiety of bacterial lipopolysaccharides.表面活性蛋白A与细菌脂多糖的脂质A部分的结合。
Biochem J. 1994 Oct 15;303 ( Pt 2)(Pt 2):407-11. doi: 10.1042/bj3030407.
7
Host defence capacities of pulmonary surfactant: evidence for 'non-surfactant' functions of the surfactant system.肺表面活性物质的宿主防御能力：表面活性物质系统“非表面活性”功能的证据。
Eur J Clin Invest. 1994 Sep;24(9):586-99. doi: 10.1111/j.1365-2362.1994.tb01110.x.
8
Targeted disruption of the surfactant protein B gene disrupts surfactant homeostasis, causing respiratory failure in newborn mice.表面活性蛋白B基因的靶向破坏会扰乱表面活性剂的稳态，导致新生小鼠呼吸衰竭。
Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7794-8. doi: 10.1073/pnas.92.17.7794.
9
Pulmonary surfactant protein A mediates enhanced phagocytosis of Mycobacterium tuberculosis by a direct interaction with human macrophages.肺表面活性蛋白A通过与人类巨噬细胞直接相互作用介导增强的结核分枝杆菌吞噬作用。
J Immunol. 1995 Dec 1;155(11):5343-51.
10
Aberrant processing of surfactant protein C in hereditary SP-B deficiency.
Am J Physiol. 1995 Apr;268(4 Pt 1):L647-56. doi: 10.1152/ajplung.1995.268.4.L647.