Guschlbauer W
CEA/Saclay, Service de Biochimie et Génétique Moléculaire, Gif-sur-Yvette, France.
Acta Biochim Pol. 1996;43(1):77-94.
This short review assembles the contributions of the author's laboratory to the structural aspects of DNA. DNA was modified by small ligands and/or substituents. There are three aspects to this work: a) Protonation of guanosine and DNA and the formation of triple- and quadruple-strands of guanosine, its nucleotides, their polymers and DNA. b) Substitution of the 2'-position of deoxyribose by the most polar atom, fluorine: studies on 2'-deoxy-2'-fluro-nucleosides, -nucleotides and their polymers, studied both by structural and biological methods. c) The effect of introducing the methyl group in the large groove of DNA: NMR studies of oligonucleotides containing N6-methylated adenine residues, and enzymatic and molecular biology work on Dam methylase are reported.
这篇简短的综述汇集了作者实验室在DNA结构方面的贡献。DNA通过小分子配体和/或取代基进行修饰。这项工作有三个方面：a）鸟苷和DNA的质子化以及鸟苷、其核苷酸、它们的聚合物和DNA的三链和四链形成。b）用极性最强的原子氟取代脱氧核糖的2'-位：通过结构和生物学方法对2'-脱氧-2'-氟核苷、核苷酸及其聚合物进行研究。c）在DNA大沟中引入甲基的影响：报道了对含有N6-甲基腺嘌呤残基的寡核苷酸的核磁共振研究，以及关于Dam甲基化酶的酶学和分子生物学工作。
