Tamaoki J, Kondo M, Tagaya E, Takemura K, Konno K
First Department of Medicine, Tokyo Women's Medical College, Japan.
Exp Lung Res. 1996 May-Jun;22(3):255-66. doi: 10.3109/01902149609031774.
The effects of Zizyphi fructus, a major constituent of Chinese anti-asthmatic herbal medicine, on ciliary motility and nitric oxide (NO) generation in canine cultured tracheal epithelium were studied by the microphoto-oscillation method and the specific amperometric method, respectively. Addition of Zizyphi fructus at 100 micrograms/mL rapidly increased ciliary beat frequency (CBF), an effect that was inhibited by NG-nitro-L-arginine methylester (L-NAME) but not by NG-nitro-D-arginine methylester (D-NAME), and this inhibition was reversed by L-arginine but not by D-arginine. Immersion of the NO-selective electrode in the medium bathing tracheal epithelial cells showed a baseline current of 21.3-60.4 pA, which corresponded to NO concentration ([NO]) at 34.4 +/- 7.8 nM. Zizyphi fructus caused a concentration-dependent increase in [NO], the maximal increase from the baseline [NO] level and the concentration of Zizyphi fructus required to produce a half-maximal effect (EC50) being 107 +/- 14 nM (p < .001) and 7.2 +/- 2.9 micrograms/mL, respectively. These results suggest that Zizyphi fructus enhances airway ciliary motility and that this effect is exerted through the stimulation of epithelial NO generation.
采用显微光振荡法和特定电流分析法，分别研究了中药抗哮喘主要成分——酸枣仁对犬培养气管上皮细胞纤毛运动和一氧化氮（NO）生成的影响。加入100微克/毫升的酸枣仁后，纤毛摆动频率（CBF）迅速增加，NG-硝基-L-精氨酸甲酯（L-NAME）可抑制此效应，而NG-硝基-D-精氨酸甲酯（D-NAME）则无此作用，L-精氨酸可逆转这种抑制，而D-精氨酸则不能。将NO选择性电极浸入气管上皮细胞的培养液中，基线电流为21.3 - 60.4 pA，对应NO浓度（[NO]）为34.4±7.8 nM。酸枣仁可使[NO]呈浓度依赖性增加，相对于基线[NO]水平的最大增加量和产生半数最大效应所需的酸枣仁浓度（EC50）分别为107±14 nM（p <.001）和7.2±2.9微克/毫升。这些结果表明，酸枣仁可增强气道纤毛运动，且该作用是通过刺激上皮细胞产生NO来实现的。
