Eckersall P D, Saini P K, McComb C
Department of Veterinary Medicine, University of Glasgow Veterinary School, UK.
Vet Immunol Immunopathol. 1996 Jun 1;51(3-4):377-85. doi: 10.1016/0165-2427(95)05527-4.
The acute phase serum protein response to infection, inflammation or trauma has been identified in a number of species and consists of alterations to the serum concentrations of several proteins. It is known that the profile of acute phase protein response to stimulation differs between species. In the pig, individual proteins have been identified as acute phase proteins in association with infection or pathological lesions. In this investigation, turpentine injection was used to stimulate a sterile inflammatory lesion in pigs so that the relative changes in acute phase protein could be determined and the most appropriate proteins identified as markers of inflammation. The mean serum concentration of the acid soluble glycoprotein fraction showed a two-fold increase with a peak 2 days after treatment. The mean serum alpha(1)-acid glycoprotein concentration fluctuated during the period following injection of turpentine with little difference from the control animals. The mean concentration of serum ceruloplasmin increased by 40% by the 4th day following treatment. The mean serum concentration of haptoglobin increased more than two-fold reaching a peak on the 2nd day after treatment. The mean serum C-reactive protein level increased eight-fold with a peak on the 2nd day after turpentine injection. C-reactive protein and haptoglobin are likely to be the best markers for the identification of inflammatory lesions in pigs.
在许多物种中都已发现了针对感染、炎症或创伤的急性期血清蛋白反应，该反应表现为几种蛋白质血清浓度的改变。已知不同物种对刺激的急性期蛋白反应特征有所不同。在猪身上，已确定个别蛋白质为与感染或病理损伤相关的急性期蛋白。在本研究中，使用松节油注射来刺激猪的无菌性炎症损伤，以便确定急性期蛋白的相对变化，并确定最适合作为炎症标志物的蛋白质。酸溶性糖蛋白组分的平均血清浓度在治疗后2天达到峰值，增加了两倍。注射松节油后的一段时间内，血清α(1)-酸性糖蛋白平均浓度波动，与对照动物差异不大。治疗后第4天，血清铜蓝蛋白的平均浓度增加了40%。触珠蛋白的平均血清浓度增加了两倍多，在治疗后第2天达到峰值。血清C反应蛋白水平增加了八倍，在注射松节油后第2天达到峰值。C反应蛋白和触珠蛋白可能是识别猪炎症损伤的最佳标志物。
