el Mzibri M, De Méo M P, Laget M, Guiraud H, Séree E, Barra Y, Duménil G
Laboratoire de Biogénotoxicologie et Mutagénèse Environnementale (EA 1784), Faculté de Pharmacie, Marseille, France.
Mutat Res. 1996 Aug 12;369(3-4):195-208. doi: 10.1016/s0165-1218(96)00052-3.
The Salmonella sulA-test is a newly developed colorimetric assay to detect genotoxins. This technique is based on the ability of DNA-damaging agents to induce the sulA gene, one of the SOS response genes. A constructed plasmid, pEM1968, carrying a fused sulA'::'lacZ was introduced into Salmonella typhimurium TA1538. Monitoring sulA gene expression was performed by assaying the beta-galactosidase activity in the transformed strain S. typhimurium TA1538/pEM1968. A simple, fast and sensitive liquid incubation procedure has been developed after optimization of the S9 mix composition and beta-galactosidase assay. The SOS-inducing potency (SOSIP, microM-1) was defined as the slopes of the non-linear dose-response relationships. Twenty-one chemicals with different modes of action were examined for a preliminary evaluation of the test. Nineteen chemicals were genotoxic in the Salmonella sulA-test. The SOSIP ranged from 1.2 x 10(-4) microM-1 (ethyl methanesulfonate) to 419.9 microM-1 (bleomycin). Sodium azide and 5-fluorouracil were not genotoxic. Frameshift, base-pair and oxidative genotoxins were detected by the tester strain. The calculated SOSIP and the minimum concentrations detected (MCD) in the Salmonella sulA-test were compared to the reported values obtained with two similar assays: the SOS Chromotest and umu-test. The SOSIP values of 12 compounds were the highest in this new assay. Five chemicals tested in the Salmonella sulA-test gave similar SOSIP values with those of one of the two other tests. ICR-191 had the highest SOSIP with the SOS Chromotest and 3-methylchloranthrene showed the highest SOSIP with the umu-test. Similarly, the lowest MCD values were found for 12 compounds in the Salmonella sulA-test. Four compounds had close MCD values in this assay and one of the two other techniques. The SOS Chromotest remained the most sensitive assay for cisplatin and ICR 191. The umu-test was the technique of choice for 3-methylchloranthrene.
沙门氏菌sulA检测是一种新开发的用于检测基因毒素的比色测定法。该技术基于DNA损伤剂诱导sulA基因（SOS反应基因之一）的能力。将携带融合的sulA'::'lacZ的构建质粒pEM1968导入鼠伤寒沙门氏菌TA1538。通过检测转化菌株鼠伤寒沙门氏菌TA1538/pEM1968中的β-半乳糖苷酶活性来监测sulA基因表达。在优化S9混合物组成和β-半乳糖苷酶测定后，开发了一种简单、快速且灵敏的液体孵育程序。SOS诱导效力（SOSIP，μM-1）定义为非线性剂量反应关系的斜率。对21种具有不同作用方式的化学物质进行了检测，以对该测试进行初步评估。19种化学物质在沙门氏菌sulA检测中具有基因毒性。SOSIP范围从1.2×10(-4) μM-1（甲磺酸乙酯）到419.9 μM-1（博来霉素）。叠氮化钠和5-氟尿嘧啶无基因毒性。移码、碱基对和氧化性基因毒素可被测试菌株检测到。将沙门氏菌sulA检测中计算出的SOSIP和检测到的最低浓度（MCD）与通过两种类似检测（SOS色测试和umu测试）获得的报告值进行比较。12种化合物的SOSIP值在这项新检测中最高。在沙门氏菌sulA检测中测试的5种化学物质与其他两项检测之一给出了相似的SOSIP值。ICR-191在SOS色测试中具有最高的SOSIP，而3-甲基氯蒽在umu测试中显示出最高的SOSIP。同样，在沙门氏菌sulA检测中，12种化合物的MCD值最低。4种化合物在该检测和其他两项技术之一中具有相近的MCD值。SOS色测试对顺铂和ICR 191仍然是最灵敏的检测方法。umu测试是检测3-甲基氯蒽的首选技术。
