Zine A, Schweitzer L
Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, KY 40292, USA.
Brain Res. 1996 May 20;721(1-2):1-10. doi: 10.1016/0006-8993(95)01496-9.
The development of Ca-ATPase immunoreactivity in gerbil outer hair cells (OHCs), assayed by immunofluorescence and postembedding immunocytochemistry, is reported here. In the adult, a linear array of label is seen inside the lateral plasma membrane. The ultrastructural distribution of Ca-ATPase near the OHC lateral plasma membrane was examined using immunogold cytochemistry and showed this calcium pumping enzyme to be present throughout the subsurface cisternal complex (SSC), especially near the innermost layers. During development, Ca-ATPase immunoreactivity appeared in patches near the lateral plasma membrane of some OHCs of the third row by 12 days after birth (DAB). By 15-16 DAB, punctate immunoreactivity was detected in the second and first rows. At 20 DAB, immunostaining near OHC lateral plasma membrane was increased, but was less continuous than OHC staining in the adult cochlea. The appearance of Ca-ATPase in OHCs coincides with the onset of auditory function and isolated OHC motility in the gerbil. The ultrastructural demonstration of abundant sites of calcium pumps in the SSC supports a role for this structure in the intracellular storage of calcium. These findings suggest a possible role of Ca-ATPase and the SSC in the regulation of slow motility of OHCs which has been reported to depend on intracellular calcium concentration.
本文报道了通过免疫荧光和包埋后免疫细胞化学方法检测沙鼠外毛细胞（OHC）中钙-ATP酶免疫反应性的发育情况。在成体中，可见外侧质膜内有一条线性排列的标记。利用免疫金细胞化学方法检测了OHC外侧质膜附近钙-ATP酶的超微结构分布，结果显示这种钙泵酶存在于整个表面下池复合体（SSC）中，尤其是最内层附近。在发育过程中，出生后12天（DAB）时，第三排一些OHC的外侧质膜附近出现了钙-ATP酶免疫反应性斑块。到15 - 16 DAB时，在第二排和第一排检测到点状免疫反应性。在20 DAB时，OHC外侧质膜附近的免疫染色增加，但不如成年耳蜗中OHC染色连续。OHC中钙-ATP酶的出现与沙鼠听觉功能和孤立OHC运动性的开始相一致。SSC中钙泵丰富位点的超微结构证明支持了该结构在细胞内钙储存中的作用。这些发现提示钙-ATP酶和SSC在调节OHC缓慢运动性中可能发挥作用，据报道OHC的缓慢运动性依赖于细胞内钙浓度。
