伊文思蓝染料和白蛋白在犬主动脉壁中的同时蓄积。

Simultaneous accumulation of Evans blue dye and albumin in the canine aortic wall.

作者信息

Fry D L, Mahley R W, Weisgraber K H, Oh S Y

出版信息

Am J Physiol. 1977 Jul;233(1):H66-79. doi: 10.1152/ajpheart.1977.233.1.H66.

DOI:10.1152/ajpheart.1977.233.1.H66

Abstract

The simultaneous transport of Evans blue dye (EBD) and 125I-labeled canine albumin (IALB) across normal and injured endothelial surface was measured by determining the intimal accumulation of EBD (ME) by a reflectometric method and accumulation of equimolar concentrations of EBD and IALB, ME was related to MI by ME = 0.092 + 5.04 MI - 0.508 MI2 for injured and ME = 0.002 + 0.435 MI (nmol-cm-2) for normal surfaces. Both ME and MI varied nonlinearly with exposure time for injured and linearly with time for normal surfaces, giving apparent permeability coefficients of 0.92 X 10(-8) cm-sec-1 for EBD and 2.00 X 10(-8) cm-sec-1 for albumin. These results show that the transvascular flux of EBD can be related to that of albumin by empirical equations, which is of practical importance but also implies unique underlying physical laws. Study of these physical laws will provide deeper insight into transvascular macromolecular transport processes.

摘要

通过反射法测定伊文思蓝染料（EBD）的内膜蓄积量（ME）以及等摩尔浓度的EBD和125I标记犬白蛋白（IALB）的蓄积量，来测量EBD和IALB同时穿过正常和受损内皮表面的情况。对于受损表面，ME与MI的关系为ME = 0.092 + 5.04 MI - 0.508 MI2；对于正常表面，ME = 0.002 + 0.435 MI（nmol·cm-2）。ME和MI在受损表面随暴露时间呈非线性变化，在正常表面随时间呈线性变化，EBD的表观渗透系数为0.92×10(-8) cm·sec-1，白蛋白为2.00×10(-8) cm·sec-1。这些结果表明，EBD的跨血管通量可通过经验方程与白蛋白的跨血管通量相关，这具有实际重要性，但也意味着独特的潜在物理规律。对这些物理规律的研究将为跨血管大分子转运过程提供更深入的见解。