Yang W, Wang X, Wang S, Yie Y, Tien P
Institute of Microbiology, Chinese Academy of Sciences, Beijing, PRC.
J Virol Methods. 1996 May;59(1-2):57-60. doi: 10.1016/0166-0934(96)02013-7.
Rice stripe virus (RSV), a planthopper-transmitted virus, was inoculated into rice protoplasts, and a one-step growth curve was determined. The amount of virus in the protoplasts decreased following the inoculation, and then increased after 8 h. The replication of RSV reached its peak 20 h after inoculation. RSV replication in the inoculated protoplasts was further demonstrated by electron microscopy.
水稻条纹病毒(RSV)是一种由稻飞虱传播的病毒,将其接种到水稻原生质体中,并测定其一步生长曲线。接种后原生质体中的病毒量减少,8小时后增加。RSV的复制在接种后20小时达到峰值。通过电子显微镜进一步证明了接种的原生质体中RSV的复制。