Chinami M, Inoue M, Masunaga K, Fukuma T, Shingu M, Toyoda T
Department of Virology, Kurume University School of Medicine, Fukuoka, Japan.
J Virol Methods. 1996 May;59(1-2):173-6. doi: 10.1016/0166-0934(96)02040-x.
Human papillomavirus (HPV) types 16 and 6b E7 proteins and their chimeric or mutant proteins were analyzed for oligonucleotide-binding activity by surface plasmon resonance-based biomolecular interaction analysis. The results indicated that type 16 E7 protein has stronger nucleic acid-binding activity than that of type 6b E7 protein. In addition, the results also indicated that the zinc finger-like motif in the C-terminal region of the type 16 E7 protein plays an important role in this activity.
通过基于表面等离子体共振的生物分子相互作用分析，对人乳头瘤病毒（HPV）16型和6b型E7蛋白及其嵌合或突变蛋白的寡核苷酸结合活性进行了分析。结果表明，16型E7蛋白比6b型E7蛋白具有更强的核酸结合活性。此外，结果还表明，16型E7蛋白C末端区域的锌指样基序在该活性中起重要作用。
