Jewers R J, Hildebrandt P, Ludlow J W, Kell B, McCance D J
Richard Dimbleby Laboratory for Cancer Virology, UMDS, St. Thomas' Hospital, London, England.
J Virol. 1992 Mar;66(3):1329-35. doi: 10.1128/JVI.66.3.1329-1335.1992.
Binding of the retinoblastoma gene product (pRB) by viral oncoproteins, including the E7 of human papillomavirus type 16 (HPV 16), is thought to be important in transformation of cells. One of the steps in transformation is the immortalization process. Here we show that mutations in E7 within the full-length genome which inhibit binding of pRB do not abrogate the ability of the HPV 16 DNA to immortalize primary human epithelial (keratinocyte) cells. A mutation in one of the cysteines of a Cys-X-X-Cys motif which is contained in the carboxy half of the E7 and is part of a zinc finger arrangement completely eliminates the ability of HPV 16 DNA to immortalize cells. The results indicate the importance of E7 in the immortalization of primary keratinocytes but suggest that the binding of pRB is not essential.
包括人乳头瘤病毒16型（HPV 16）E7蛋白在内的病毒癌蛋白与视网膜母细胞瘤基因产物（pRB）的结合，被认为在细胞转化过程中起着重要作用。细胞转化的其中一个步骤是永生化过程。在此我们表明，全长基因组中抑制pRB结合的E7突变，并不会消除HPV 16 DNA使原代人上皮（角质形成）细胞永生化的能力。E7蛋白羧基端含有一个Cys-X-X-Cys基序，该基序中的一个半胱氨酸发生突变，此半胱氨酸是锌指结构的一部分，这会完全消除HPV 16 DNA使细胞永生化的能力。结果表明E7在原代角质形成细胞永生化过程中具有重要作用，但也表明pRB的结合并非必不可少。
