利用绿色荧光蛋白监测基因工程菌在水生环境中的存活情况。
Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments.
作者信息
Leff L G, Leff A A
机构信息
Department of Biological Sciences, Kent State University, Ohio 44242, USA.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3486-8. doi: 10.1128/aem.62.9.3486-3488.1996.
Abstract
Many methods for detecting model genetically engineered microorganisms (GEMs) in experimental ecosystems rely on cultivation of introduced cells. In this study, survival of Escherichia coli was monitored with the green fluorescent protein (GFP) gene. This approach allowed enumeration of GEMs by both plating and microscopy. Use of the GFP-marked GEMs revealed that E. coli persisted in stream water at higher densities as determined microscopically than as determined by CFU enumeration. The GFP gene did not negatively impact the fitness of the host strain.
摘要
许多用于检测实验生态系统中模型基因工程微生物（GEMs）的方法都依赖于引入细胞的培养。在本研究中，利用绿色荧光蛋白（GFP）基因监测了大肠杆菌的存活情况。这种方法能够通过平板计数和显微镜观察对GEMs进行计数。使用GFP标记的GEMs结果显示，通过显微镜观察确定，大肠杆菌在溪水中以比通过菌落形成单位（CFU）计数所确定的更高密度持续存在。GFP基因对宿主菌株的适应性没有负面影响。
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