通过大肠杆菌K-12在葡萄糖控制的高细胞密度发酵中过量生产来自聚球藻属菌株PCC6301的重组1,5-二磷酸核酮糖羧化酶/加氧酶。
Overproduction of recombinant ribulose 1,5-bisphosphate carboxylase/oxygenase from Synechococcus sp. strain PCC6301 in glucose-controlled high-cell-density fermentations by Escherichia coli K-12.
作者信息
Kleman G L, Horken K M, Tabita F R, Strohl W R
机构信息
Department of Microbiology, Ohio State University, Columbus 43210, USA.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3502-7. doi: 10.1128/aem.62.9.3502-3507.1996.
Abstract
A predictive and feedback glucose feed controller, previously developed for nutrient-sufficient growth of Escherichia coli to high cell densities, was used to produce large quantities of a heterologous, cyanobacterial recombinant hexadecameric (L8S8) protein, ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) in E. coli. Culture and plasmid stability conditions were optimized to yield the production of approximately 1 g of soluble, active recombinant RubisCO per liter. Recombinant RubisCO also was produced in lactose-induced high-cell-density fermentation of E. coli K-12.
摘要
一种先前开发用于使大肠杆菌在营养充足的条件下生长至高密度的预测性和反馈性葡萄糖进料控制器，被用于在大肠杆菌中大量生产一种异源的蓝细菌重组十六聚体（L8S8）蛋白，即1,5-二磷酸核酮糖羧化酶/加氧酶（RubisCO）。优化了培养和质粒稳定性条件，以实现每升产生约1克可溶性、活性重组RubisCO。重组RubisCO也在大肠杆菌K-12的乳糖诱导高细胞密度发酵中产生。
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