通过聚合酶链反应检测供体角膜培养基中的单纯疱疹病毒DNA。
Detection of herpes simplex virus DNA in donor cornea culture medium by polymerase chain reaction.
作者信息
Morris D J, Cleator G M, Klapper P E, Cooper R J, Biney E O, Dennett C, Marcyniuk B, Tullo A B
机构信息
Department of Pathological Sciences, Medical School, University of Manchester.
出版信息
Br J Ophthalmol. 1996 Jul;80(7):654-7. doi: 10.1136/bjo.80.7.654.
AIMS/BACKGROUND: Herpes simplex virus (HSV) may establish latent infection in the cornea and therefore be transmissible by corneal transplantation. Monitoring of donor cornea culture medium was evaluated for HSV infection.
METHODS
HSV was sought using virus isolation in cell culture, and its DNA was amplified to detectable levels using the polymerase chain reaction (PCR).
RESULTS
Virus isolation in cell culture was negative on neat, cell pellet, and cell free supernatant prepared from the spent culture media of 80 corneas. Three cell pellets (3.8%) were positive for HSV DNA. The PCR positive culture negative results might have reflected latent rather than active HSV infection of the cornea. Post transplant follow up of the three recipients of corneas with HSV PCR positive organ culture media revealed no evidence of HSV induced eye disease or primary graft failure.
CONCLUSION
Screening of corneal culture medium for HSV by virus culture or for HSV DNA by PCR could not be recommended.
目的/背景：单纯疱疹病毒（HSV）可在角膜中建立潜伏感染，因此可通过角膜移植传播。对供体角膜培养基进行监测以评估HSV感染情况。
方法
采用细胞培养中的病毒分离法寻找HSV，并使用聚合酶链反应（PCR）将其DNA扩增至可检测水平。
结果
从80个角膜的用过的培养基中制备的原液、细胞沉淀和无细胞上清液在细胞培养中的病毒分离结果均为阴性。三个细胞沉淀（3.8%）的HSV DNA呈阳性。PCR阳性而培养阴性的结果可能反映了角膜的潜伏性而非活动性HSV感染。对三名接受HSV PCR阳性器官培养基角膜移植的受者进行移植后随访，未发现HSV诱发眼病或原发性移植失败的证据。
结论
不建议通过病毒培养筛查角膜培养基中的HSV或通过PCR筛查HSV DNA。
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