McElhinney L M, Cooper R J, Morris D J
Department of Pathological Sciences, University of Manchester, UK.
J Virol Methods. 1995 Jun;53(2-3):223-33. doi: 10.1016/0166-0934(95)00019-q.
Human cytomegalovirus and human herpesvirus-6 are closely related viruses which cause similar diseases, have similar cellular repositories of latent infection, and may be detected largely in the same types of clinical specimens. DNA amplification appears likely to play an increasing role in the diagnosis of recent and remote infection with these agents. A sensitive multiplex polymerase chain reaction was therefore developed for the two viruses and for human beta-globin DNA. Optimization of parameters such as the primers, primer concentrations, magnesium concentration, and buffer constituents was crucial in achieving a sensitive assay. Preliminary results indicated that the assay could simultaneously monitor DNA extraction from clinical specimens and allow detection of HCMV or HHV-6 in patients with diseases possibly caused by either pathogen.
人巨细胞病毒和人疱疹病毒6型是密切相关的病毒，它们引起相似的疾病，具有相似的潜伏感染细胞储存库，并且在很大程度上可以在相同类型的临床标本中检测到。DNA扩增似乎在这些病原体近期和远期感染的诊断中发挥着越来越重要的作用。因此，针对这两种病毒以及人β-珠蛋白DNA开发了一种灵敏的多重聚合酶链反应。优化引物、引物浓度、镁离子浓度和缓冲液成分等参数对于实现灵敏的检测至关重要。初步结果表明，该检测方法可以同时监测临床标本的DNA提取情况，并能够检测出可能由这两种病原体之一引起疾病的患者体内的人巨细胞病毒或人疱疹病毒6型。
