Abruzzese E, Buss D, Rainer R, Rao P N, Pettenati M J
Department of Pediatrics, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1076, USA.
Leuk Res. 1996 Jul;20(7):551-7. doi: 10.1016/0145-2126(95)00130-1.
The lineage involvement in myelodysplastic syndromes (MDS) is still unclear. To determine the clonality and the evolution of the disorder, a retrospective study on bone marrow smears from seven MDS patients with trisomy 8 was performed using fluorescence in situ hybridization (FISH). We observed that the trisomy of chromosome 8 was selectively expressed in the myeloid-derived cells. No mature lymphocytes or plasma cells expressed three signals. Our studies demonstrate here the value of FISH for identifying the affected cell lineage. Furthermore, the easy quantification of the abnormal cells can help in assessing the progression of the disease.
骨髓增生异常综合征（MDS）中的谱系受累情况仍不明确。为了确定该疾病的克隆性及演变，我们使用荧光原位杂交（FISH）技术对7例8号染色体三体的MDS患者的骨髓涂片进行了回顾性研究。我们观察到8号染色体三体在髓系来源的细胞中选择性表达。未发现成熟淋巴细胞或浆细胞表达三个信号。我们的研究在此证明了FISH在识别受累细胞谱系方面的价值。此外，对异常细胞的轻松定量有助于评估疾病的进展。
