Doucet-Populaire F, Lalande V, Carpentier E, Bourgoin A, Dailloux M, Bollet C, Vachée A, Moinard D, Texier-Maugein J, Carbonnelle B, Grosset J
Hôpital Pitié-Salpétrière, Paris, France.
Tuber Lung Dis. 1996 Aug;77(4):358-62. doi: 10.1016/s0962-8479(96)90102-1.
Nine French laboratories routinely involved in mycobacterial work.
To assess the detection of Mycobacterium tuberculosis in experimental samples by polymerase chain reaction (PCR) using the insertion sequence IS6110 as a target for deoxyribonucleic acid (DNA) amplification.
Nine laboratories participated in a blind study of the detection of M. tuberculosis by PCR in 20 coded samples containing either a definite number of M. tuberculosis complex (positive samples) or environmental mycobacteria (four samples) or no mycobacteria (five samples).
Five laboratories reported false-positive PCR results, with an average rate of 7%. All laboratories except one reported positive PCR results for samples containing 10(5) cfu/ml or more. M. tuberculosis DNA was detected in two thirds of samples containing 10(4) and 10(3) cfu/ml, and in one third of the samples containing 10(2) cfu/ml.
The results of the study suggest that PCR using IS6110 as a target for DNA amplication is neither very sensitive nor really specific for the detection of M. tuberculosis.
九家法国实验室常规参与分枝杆菌相关工作。
评估以插入序列IS6110作为脱氧核糖核酸（DNA）扩增靶点，通过聚合酶链反应（PCR）检测实验样本中结核分枝杆菌的情况。
九家实验室参与了一项盲法研究，对20份编码样本进行结核分枝杆菌的PCR检测，这些样本中包含一定数量的结核分枝杆菌复合群（阳性样本）、环境分枝杆菌（4份样本）或不含分枝杆菌（5份样本）。
五家实验室报告了假阳性PCR结果，平均发生率为7%。除一家实验室外，所有实验室均报告了含10⁵ cfu/ml及以上的样本PCR结果为阳性。在含10⁴和10³ cfu/ml的样本中，三分之二检测到结核分枝杆菌DNA，在含10² cfu/ml的样本中，三分之一检测到结核分枝杆菌DNA。
研究结果表明，以IS6110作为DNA扩增靶点的PCR检测结核分枝杆菌既不太敏感也不太特异。
