基于IS6110的结核分枝杆菌复合群DNA指纹图谱及诊断技术的特异性
Specificity of IS6110-based DNA fingerprinting and diagnostic techniques for Mycobacterium tuberculosis complex.
作者信息
Githui W A, Wilson S M, Drobniewski F A
机构信息
PHLS Mycobacterium Reference Unit, Dulwich Public Health Laboratory and Department of Microbiology, Kings College School of Medicine and Dentistry, Dulwich Hospital, London, United Kingdom.
出版信息
J Clin Microbiol. 1999 Apr;37(4):1224-6. doi: 10.1128/JCM.37.4.1224-1226.1999.
Abstract
Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific primers yielded a 181-bp fragment only in DNA from M. tuberculosis complex isolates.
摘要
用源自IS6110的探针，对从结核分枝杆菌、非结核分枝杆菌和非分枝杆菌属物种中提取的DNA进行限制性片段长度多态性分析和杂交，证实IS6110对结核分枝杆菌复合群具有特异性。此外，用IS6110特异性引物进行DNA扩增，仅在结核分枝杆菌复合群分离株的DNA中产生一个181bp的片段。
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