评估改良半固体Rappaport-Vassiliadis培养基（MSRV）上的运动性富集以及结合Rambach琼脂的自动电导率用于奶粉厂环境样品中沙门氏菌的检测。
Evaluation of motility enrichment on modified semisolid Rappaport-Vassiliadis medium (MSRV) and automated conductance in combination with Rambach agar for Salmonella detection in environmental samples of a milk powder factory.
作者信息
Joosten H M, van Dijck W G, van der Velde F
机构信息
Coberco Research, Deventer, The Netherlands.
出版信息
Int J Food Microbiol. 1994 May;22(2-3):201-6. doi: 10.1016/0168-1605(94)90142-2.
The efficacy of motility enrichment on modified semisolid Rappaport-Vassiliadis medium (MSRV) and an automated conductance method for the detection of Salmonella in environmental samples was evaluated. Two hundred and ten environmental samples from unrestricted areas of a milk powder factory, 49 of which were artificially contaminated with Salmonella infantis, were examined. From exactly 100 samples Salmonella could be isolated. With the conventional (ISO-DIS 6579) method a 100% score was obtained, whereas the MSRV method gave 82 positive results. The conductance method permitted the detection of Salmonella in only 66 samples. The use of Rambach agar improved isolation efficiency of Salmonella from enrichment broths.
评估了在改良半固体Rappaport-Vassiliadis培养基（MSRV）上进行动力富集以及采用自动电导法检测环境样品中沙门氏菌的效果。对一家奶粉厂非限制区域的210份环境样品进行了检测，其中49份被婴儿沙门氏菌人工污染。从恰好100份样品中分离出了沙门氏菌。采用传统方法（ISO-DIS 6579）的检出率为100%，而MSRV方法得到了82个阳性结果。电导法仅能检测出66份样品中的沙门氏菌。使用Rambach琼脂提高了从增菌肉汤中分离沙门氏菌的效率。
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