Pollard J K, Mitchell M D
Department of Obstetrics and Gynecology, University of Utah, Salt Lake City, USA.
J Matern Fetal Med. 1996 Mar-Apr;5(2):93-8. doi: 10.1002/(SICI)1520-6661(199603/04)5:2<93::AID-MFM9>3.0.CO;2-L.
The objective of this study was to evaluate the effects of known stimulants of prostaglandin production on cultured myometrial cells from women who are at various gestational ages. To evaluate this, myometrial segments were obtained from 12 patients at 27-40 weeks gestation at cesarean delivery. Myometrial cells were then isolated and grown to confluence in culture. Incubations were conducted in quadruplicate for 16 hours with interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA), or ionomycin. Prostaglandins E2, F2 alpha, and 6-keto F1 alpha (the stable metabolite of prostacyclin) were measured by radioimmunoassay and cellular protein determined. The results revealed that cultured myometrial cells produced prostaglandins in response to IL-1 beta, TNF-alpha, EGF, PMA, and ionomycin at all gestational ages tested at a significantly increased rate (P < 0.05 vs controls at and above 10 ng/ml of IL-1 beta, TNF alpha, and EGF; 1 microM for PMA, and 5 microM for ionomycin). The major prostaglandin produced in response to each stimulant was 6-keto-PGF1 alpha. Cultured human myometrial cells respond to known stimulants of prostaglandin production throughout the third trimester of pregnancy in the absence of labor. The myometrium may therefore be a source of prostaglandins that influences uterine activity.
本研究的目的是评估已知的前列腺素生成刺激物对来自不同孕周女性的培养子宫肌层细胞的影响。为评估此影响，在剖宫产时从12例妊娠27 - 40周的患者获取子宫肌层组织块。然后分离子宫肌层细胞并在培养中生长至汇合。分别用白细胞介素 - 1β（IL - 1β）、肿瘤坏死因子 - α（TNF - α）、表皮生长因子（EGF）、佛波酯12 - 肉豆蔻酸13 - 乙酸酯（PMA）或离子霉素进行一式四份的孵育16小时。通过放射免疫测定法测量前列腺素E2、F2α和6 - 酮F1α（前列环素的稳定代谢产物）并测定细胞蛋白。结果显示，在所有测试的孕周，培养的子宫肌层细胞对IL - 1β、TNF - α、EGF、PMA和离子霉素产生前列腺素的速率显著增加（与10 ng/ml及以上的IL - 1β、TNF - α和EGF；1 μM的PMA以及5 μM的离子霉素的对照组相比，P < 0.05）。对每种刺激物产生的主要前列腺素是6 - 酮 - PGF1α。在无分娩的情况下，培养的人子宫肌层细胞在妊娠晚期对已知的前列腺素生成刺激物有反应。因此，子宫肌层可能是影响子宫活动的前列腺素来源。
