Pollard J K, Mitchell M D
Department of Obstetrics and Gynecology, University of Utah, Salt Lake City, USA.
Am J Obstet Gynecol. 1996 Feb;174(2):682-6. doi: 10.1016/s0002-9378(96)70450-7.
Our purpose was to evaluate the effects of known stimulants of prostaglandin production on cultured myometrial cells from women in labor with and without intrauterine infection.
Myometrial segments were obtained from 16 patients between 33 and 40 weeks' gestation who had been in labor for > or = 8 hours at cesarean delivery; 8 patients had clinical chorioamnionitis and 8 did not. Myometrial cells were isolated and grown in culture. Incubations were conducted with interleukin-1 beta, tumor necrosis factor-alpha, or epidermal growth factor. Prostaglandin E2, prostaglandin F2 alpha, and 6-keto-prostaglandin F1 alpha (the stable metabolite of prostacyclin) were measured by radioimmunoassay, and cellular protein was determined.
Cultured human myometrial cells from patients with and without prior intrauterine infection produced prostaglandins in response to interleukin-1 beta, tumor necrosis factor-alpha, and epidermal growth factor at a significantly increased rate (p<0.05 vs controls at and above 10 ng/ml of interleukin-1 beta, tumor necrosis factor-alpha, and epidermal growth factor). The major prostaglandin produced in response to each stimulant was 6-keto-prostaglandin F1 alpha; however, this response was attenuated in cells from patients with intrauterine infection.
Cultured human myometrial cells from patients with and without prior intrauterine infection respond to known stimulants of prostaglandin production. Prior intrauterine infection has no effect on baseline prostaglandin production, but the amount of prostacyclin produced as a response to cellular stimulants is decreased with prior intrauterine infection. This effect may have a role in regulating myometrial function in intrauterine infection.
我们的目的是评估已知的前列腺素生成刺激物对有或无宫内感染的分娩期女性子宫肌层细胞培养物的影响。
从16例妊娠33至40周、剖宫产时已分娩≥8小时的患者获取子宫肌层组织；8例患者有临床绒毛膜羊膜炎，8例没有。分离子宫肌层细胞并进行培养。用白细胞介素-1β、肿瘤坏死因子-α或表皮生长因子进行孵育。通过放射免疫分析法测定前列腺素E2、前列腺素F2α和6-酮-前列腺素F1α（前列环素的稳定代谢产物），并测定细胞蛋白。
有或无既往宫内感染患者的培养人子宫肌层细胞对白细胞介素-1β、肿瘤坏死因子-α和表皮生长因子产生前列腺素的速率显著增加（白细胞介素-1β、肿瘤坏死因子-α和表皮生长因子浓度为10 ng/ml及以上时，与对照组相比p<0.05）。对每种刺激物产生的主要前列腺素是6-酮-前列腺素F1α；然而，宫内感染患者细胞中的这种反应减弱。
有或无既往宫内感染患者的培养人子宫肌层细胞对已知的前列腺素生成刺激物有反应。既往宫内感染对基线前列腺素生成无影响，但既往宫内感染会使作为对细胞刺激物反应而产生的前列环素量减少。这种效应可能在调节宫内感染时的子宫肌层功能中起作用。
