Shcherbakova E G, Rastunova G A, Medvedeva M M, Zhuravleva T P, Kuchushev Sh A
Antibiotiki. 1977 May;22(5):413-7.
The effect of gentamicin on the functional state of the cells of the reticuloendothelial system (RES) of the abdominal cavity of mouse macrophages and the cell culture of the rabbit embryon kidneys was studied. Gentamicin administered intramuscularly in multiple doses of I and 10 mg/kg for 6--14 days did not induce significant changes in the absorptive activity of the RES. In doses of 20 and 40 mg/kg gentamicin induced some inhibition of the absorptive activity of the abdominal cavity macrophages, which was accompanied by a decrease in the content of the total protein and an increase in the activity of the acid phosphatase in the cell cytoplasm. The changes were reversible and persisted for not more than 48 hours after the last administration of the antibiotic. Gentamicin inhibited the cell growth in the culture of the rabbit embryon kidneys in concentrations higher than 1000 mg/kg, LD50 of gentamicin for mice weighing 20--22 gm being 244 mg/kg. Preliminary administration of amigluracyl provided a decrease in the toxic effect of gentamicin in both the cell culture and the host, which was possibly associated with the anabolyzing effect of amigluracyl.
研究了庆大霉素对小鼠腹腔巨噬细胞和兔胚胎肾细胞培养物中网状内皮系统（RES）细胞功能状态的影响。以1和10mg/kg的多剂量肌肉注射庆大霉素，持续6 - 14天，未引起RES吸收活性的显著变化。当剂量为20和40mg/kg时，庆大霉素对腹腔巨噬细胞的吸收活性有一定抑制作用，同时伴有细胞质中总蛋白含量降低和酸性磷酸酶活性增加。这些变化是可逆的，在最后一次使用抗生素后持续不超过48小时。当庆大霉素浓度高于1000mg/kg时，可抑制兔胚胎肾细胞培养物中的细胞生长，体重20 - 22g小鼠的庆大霉素半数致死量（LD50）为244mg/kg。预先给予阿米鲁胺可降低庆大霉素在细胞培养物和宿主体内的毒性作用，这可能与阿米鲁胺的合成代谢作用有关。
