表达人生长激素释放因子基因的转基因小鼠垂体中“增生-腺瘤序列”的免疫组织化学特征

Immunohistochemical characterization of "hyperplasia-adenoma sequence" in the pituitaries of transgenic mice expressing a human growth hormone-releasing factor gene.

作者信息

Umemura S, Oda K, Utsunomiya H, Sanno N, Itoh J, Katakami H, Osamura R Y

机构信息

Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan.

出版信息

Tokai J Exp Clin Med. 1995 Jul;20(2):71-9.

PMID:8797263

Abstract

The morphology of hyperplastic pituitaries in seven human growth hormone-releasing factor (hGRF) transgenic mice were compared to those of two normal control mice. Under continuous stimulation by hGRF, both the total volume of the pituitary and the size of individual cells increased, and a nodular lesion, designated a "hypertrophic nodule", was identified. Immunohistochemically, the hyperplastic pituitaries consisted of various numbers of cells immunoreactive for rGH, rPRL, hACTH, rLH beta, hFSH beta, and r alpha SU, whereas the "hypertrophic nodule" was composed of rGH, rPRL, and rTSH beta positive cells, similar to the adenoma. The presence of the "hypertrophic nodule", which was intermediate in appearance between the controls and the adenomas, suggests a close relation between continuous hGRF stimulation and the development of a hyperplasia-adenoma sequence in the pituitary.

摘要

将七只人类生长激素释放因子（hGRF）转基因小鼠的增生垂体形态与两只正常对照小鼠的垂体形态进行了比较。在hGRF的持续刺激下，垂体的总体积和单个细胞的大小均增加，并发现了一个结节性病变，称为“肥大结节”。免疫组织化学显示，增生的垂体由各种数量的对rGH、rPRL、hACTH、rLHβ、hFSHβ和rαSU呈免疫反应的细胞组成，而“肥大结节”由rGH、rPRL和rTSHβ阳性细胞组成，类似于腺瘤。“肥大结节”的存在，其外观介于对照和腺瘤之间，提示hGRF持续刺激与垂体增生-腺瘤序列的发展之间存在密切关系。