Verburg-van Kemenade B M, Daly J G, Groeneveld A, Wiegertjes G F
Department of Experimental Animal Morphology and Cell Biology, Wageningen Agricultural University, Netherlands.
Vet Immunol Immunopathol. 1996 May;51(1-2):189-200. doi: 10.1016/0165-2427(95)05506-1.
Normal carp serum contains inhibitory and stimulatory factors for macrophage and neutrophilic granulocyte respiratory burst activity. As stimulatory factors were only effective in combination with phorbol myristate actetate (PMA) activation, it is concluded that they are probably linked to protein kinase C activation. Both the stimulatory and inhibitory factors are heat stable. Macrophage- and neutrophilic granulocyte-enriched cell fractions from the pronephros of carp had high respiratory burst- and high bactericidal in vitro responses to virulent atypical Aeromonas salmonicida bacteria. Serum factors were inhibitory for the A. salmonicida induced respiratory burst activity. No change in inhibitory or stimulatory serum factors could be observed during a 12-day challenge experiment with A. salmonicida, or during a rechallenge of survivors from a previous sub-lethal infection. The sensitivity of macrophages and neutrophilic granulocytes to stimulation of respiratory burst activity by PMA was not significantly altered. Culture supernatants from PHA pre-treated lymphocytes stimulated the respiratory burst activity of macrophages and neutrophilic granulocytes suggesting that serum factors may partially be lymphocyte derived.
正常鲤鱼血清含有针对巨噬细胞和嗜中性粒细胞呼吸爆发活性的抑制因子和刺激因子。由于刺激因子仅在与佛波酯（PMA）激活联合使用时才有效，因此得出结论，它们可能与蛋白激酶C激活有关。刺激因子和抑制因子均具有热稳定性。来自鲤鱼前肾的富含巨噬细胞和嗜中性粒细胞的细胞组分对强毒非典型杀鲑气单胞菌具有高呼吸爆发和高体外杀菌反应。血清因子对杀鲑气单胞菌诱导的呼吸爆发活性具有抑制作用。在对杀鲑气单胞菌进行的为期12天的攻毒实验期间，或在对先前亚致死感染的幸存者进行再次攻毒期间，未观察到抑制性或刺激性血清因子的变化。巨噬细胞和嗜中性粒细胞对PMA刺激呼吸爆发活性的敏感性没有明显改变。PHA预处理淋巴细胞的培养上清液刺激了巨噬细胞和嗜中性粒细胞的呼吸爆发活性，这表明血清因子可能部分源自淋巴细胞。
