[大环内酯类药物对人全血模型中细胞因子mRNA表达的影响]

[Effect of macrolides on cytokine mRNA expression in human whole blood model].

作者信息

Arikawa K, Honda J, Sugihara E, Kuwamoto M, Kumagai M, Akiyoshi H, Matsumoto K, Shiraishi T, Rikimaru T, Oizumi K

机构信息

First Department of Internal Medicine, Kurume University School of Medicine.

出版信息

Kansenshogaku Zasshi. 1996 Jul;70(7):696-701. doi: 10.11150/kansenshogakuzasshi1970.70.696.

DOI:10.11150/kansenshogakuzasshi1970.70.696

PMID:8797304

Abstract

Recently, low dose and long term use of Macrolides (Mls) has been reported to be effective in treatment of chronic lower respiratory tract infections, however its mechanism is still obscure. We evaluated the effect of Mls (EM, AZM, RKM) on cytokine mRNA expressions. We preincubated the whole blood with several concentrations of Mls and removed the Mls and then stimulated human whole blood with LPS as an experimental vivo model. In order to examine cytokine mRNA expressions, we used the RT-PCR method. Cytokine mRNA expressions were suppressed significantly (p < 0.05) by pretreatment with EM, AZM; moreover, the suppression was peaked at low concentrations (0.04 approximately 0.2 microgram/ml). Although, Cytokine mRNA expressions were not suppressed by pretreatment with RKM. These results suggest that EM, AZM have suppression on Cytokine mRNA expressions, and consequently, this suppression has a reasonable effect for DPB patients.

摘要

最近，有报道称低剂量长期使用大环内酯类药物（Mls）对慢性下呼吸道感染有效，但其作用机制仍不清楚。我们评估了Mls（依托红霉素、阿奇霉素、克拉霉素）对细胞因子mRNA表达的影响。我们将全血与几种浓度的Mls预孵育，去除Mls后，以脂多糖刺激人全血作为体内实验模型。为检测细胞因子mRNA表达，我们采用逆转录聚合酶链反应（RT-PCR）法。依托红霉素、阿奇霉素预处理可显著抑制细胞因子mRNA表达（p<0.05）；此外，抑制作用在低浓度（0.04至0.2微克/毫升）时达到峰值。虽然克拉霉素预处理未抑制细胞因子mRNA表达。这些结果表明，依托红霉素、阿奇霉素对细胞因子mRNA表达有抑制作用，因此，这种抑制作用对弥漫性泛细支气管炎（DPB）患者有合理的疗效。