Okubo Y
Department of Medicine, Kurume University School of Medicine, Japan.
Kurume Med J. 1997;44(2):115-23. doi: 10.2739/kurumemedj.44.115.
Several reports described a favorable effect of "low-dose and long-term" erythromycin (EM) on chronic obstructive pulmonary diseases including diffuse panbronchiolitis (DPB), although its mechanism still remains obscure. We investigated the effect of some macrolides, erythromycin (EM), rokitamycin (RKM), midecamycin (MDM) on the expression of neutrophil adhesion molecule Mac-1 using LPS-stimulated human whole blood as an experimental vivo model. Samples from six healthy volunteer were treated with various concentrations (0.02 ug/ml-20 ug/ml) of EM, RKM and MDM for 1 to 3 hs. Surface expression of Mac-1 antigen was determined by use of flow-cytometry. When pretreated with EM and MDM for 1 and 3 hs, significant reduction in Mac-1 expression was observed, but with RKM no substantial reduction. These findings indicate that some macrolides such as EM suppress the surface expression of Mac-1 on neutrophil and may alleviate local alveolar injury in chronic pulmonary diseases.
几份报告描述了“低剂量长期使用”红霉素（EM）对包括弥漫性泛细支气管炎（DPB）在内的慢性阻塞性肺疾病有积极作用，但其机制仍不清楚。我们以脂多糖刺激的人全血作为实验体内模型，研究了几种大环内酯类药物，红霉素（EM）、罗他霉素（RKM）、麦迪霉素（MDM）对中性粒细胞粘附分子Mac-1表达的影响。来自六名健康志愿者的样本用不同浓度（0.02μg/ml - 20μg/ml）的EM、RKM和MDM处理1至3小时。通过流式细胞术测定Mac-1抗原的表面表达。当用EM和MDM预处理1小时和3小时时，观察到Mac-1表达显著降低，但用RKM处理则没有明显降低。这些发现表明，一些大环内酯类药物如EM可抑制中性粒细胞上Mac-1的表面表达，并可能减轻慢性肺部疾病中的局部肺泡损伤。
