Griffin L F, Calder J A
Appl Environ Microbiol. 1977 May;33(5):1092-6. doi: 10.1128/aem.33.5.1092-1096.1977.
The water-soluble fractions of three crude and two refined oils reduced the growth rate and maximum cell density of the marine bacterium Serratia marinorubra grown in batch culture. The weathering of a crude and a refined oil was simulated in the laboratory. The water-soluble fractions remaining from this process were more toxic to S. marinorubra than were the parent unweathered oils. Increases in the magnitude of toxic effect of 3 to 30 times were observed as a function of decreasing the concentration of yeast extract in the cultures from 0.1 to 0.05 and 0.01%. The toxicity did not correlate with the concentration of total water-soluble fraction or of aromatic hydrocarbons in the water-soluble fraction. Affected cultures did not exhibit a residual toxicity after being back-inoculated into control media.
三种原油和两种精炼油的水溶性馏分降低了分批培养的海洋细菌海红红球菌的生长速率和最大细胞密度。在实验室模拟了一种原油和一种精炼油的风化过程。该过程中剩余的水溶性馏分对海红红球菌的毒性比未风化的母油更大。随着培养物中酵母提取物浓度从0.1%降至0.05%和0.01%,观察到毒性效应的幅度增加了3至30倍。毒性与水溶性馏分中总水溶性馏分或芳烃的浓度无关。受影响的培养物重新接种到对照培养基中后未表现出残留毒性。