Hult K, Hökby E, Gatenbeck S
Appl Environ Microbiol. 1977 Jun;33(6):1275-7. doi: 10.1128/aem.33.6.1275-1277.1977.
A method is described for ochratoxin B analysis, which is adapted to the earlier described method of ochratoxin A analysis, using carboxypeptidase A (K. Hult and S. Gatenbeck, J. Assoc. Off. Anal. Chem. 59:128-129, 1976). The fluorescence spectra of ochratoxins A and B coincide too much to allow direct discrimination of the two compounds. A method using the differences in kinetic parameters of the enzymatic hydrolysis of the two compounds is suggested for the analysis of mixtures of the ochratoxins.
本文描述了一种赭曲霉毒素B的分析方法,该方法是在先前所述的赭曲霉毒素A分析方法基础上改进而来,采用了羧肽酶A(K. 胡尔特和S. 加滕贝克,《官方分析化学师协会杂志》59:128 - 129, 1976)。赭曲霉毒素A和B的荧光光谱过于相似,无法直接区分这两种化合物。建议采用一种利用两种化合物酶促水解动力学参数差异的方法来分析赭曲霉毒素混合物。