Hult K, Hökby E, Hägglund U, Gatenbeck S, Rutqvist L, Sellyey G
Appl Environ Microbiol. 1979 Nov;38(5):772-6. doi: 10.1128/aem.38.5.772-776.1979.
A procedure is presented for screening the quality of feed in respect to ochratoxin A contamination based upon the analysis of ochratoxin A in pig blood. Representative samples from large feed lots may be obtained by using pigs as in vivo sample collectors which enrich the toxin and forms homogeneous samples in the blood. The spectrofluorometric procedure for ochratoxin A analysis (K. Hult and S. Gatenbeck, J. Assoc. Off. Anal. Chem. 59:128-129, 1976) has been adapted to pig blood and has been simplified to involve only three extraction steps. A volume of 2.5 ml of blood or plasma is needed, and the detection limit is 2 ng of ochratoxin A per ml. The disappearance of ochratoxin A from pig blood as a function of time has been studied. A feeding experiment with ochratoxin A has been performed, and the time course of the concentration of ochratoxin A in blood has been followed during the experiment.
本文介绍了一种基于猪血液中赭曲霉毒素A的分析来筛查饲料中赭曲霉毒素A污染质量的方法。大型饲料场的代表性样本可通过将猪用作体内样本采集器来获得，猪能富集毒素并在血液中形成均匀样本。用于赭曲霉毒素A分析的荧光分光光度法（K. 胡尔特和S. 加滕贝克，《官方分析化学家协会杂志》59:128 - 129, 1976）已适用于猪血液，并简化为仅涉及三个提取步骤。需要2.5毫升血液或血浆，检测限为每毫升2纳克赭曲霉毒素A。研究了赭曲霉毒素A在猪血液中随时间的消失情况。进行了一项关于赭曲霉毒素A的喂养实验，并在实验过程中跟踪了血液中赭曲霉毒素A浓度的时间进程。
