Yamamoto A, Shimizu E, Ogura T, Sone S
Third Department of Internal Medicine, Tokushima University School of Medicine, Japan.
Int J Cancer. 1996 Aug 22;69(4):283-9. doi: 10.1002/(SICI)1097-0215(19960822)69:4<283::AID-IJC8>3.0.CO;2-T.
Auto-antibodies against L-myc oncogene products (L-Myc) in sera from lung cancer patients were examined using bacterially synthesized glutathione S-transferase (GST) L-Myc fusion proteins and Western blot analysis. The detection rate of anti-L-Myc antibodies in sera from lung cancer patients was 10%, while that in sera obtained from normal volunteers was 0%. Five patients with non-small-cell lung cancers (2 adenocarcinomas, 2 squamous-cell carcinomas and I large-cell carcinoma) were included in the group with anti-L-Myc antibodies. These auto-antibodies belonged to the IgG class and recognized the carboxy terminus of L-Myc. Circulating L-Myc was not detected in sera from patients with anti-L-Myc antibodies. Differences in age, sex, performance status, histology, stage, smoking history and prior treatment were not significantly different between anti-L-Myc antibody-positive and antibody-negative patients. Anti-nuclear antibodies were detected in 40% of lung cancer patients and 57% of those with anti-L-Myc antibodies. Our data suggest that detection of anti-L-Myc antibodies may be helpful in the diagnosis and evaluation of the host-immune response to L-Myc in a subset of lung cancer patients.
使用细菌合成的谷胱甘肽S-转移酶（GST）L-Myc融合蛋白和蛋白质印迹分析，检测肺癌患者血清中针对L-myc癌基因产物（L-Myc）的自身抗体。肺癌患者血清中抗L-Myc抗体的检出率为10%，而正常志愿者血清中的检出率为0%。抗L-Myc抗体组包括5例非小细胞肺癌患者（2例腺癌、2例鳞状细胞癌和1例大细胞癌）。这些自身抗体属于IgG类，识别L-Myc的羧基末端。在抗L-Myc抗体患者的血清中未检测到循环L-Myc。抗L-Myc抗体阳性和抗体阴性患者在年龄、性别、体能状态、组织学、分期、吸烟史和既往治疗方面的差异无统计学意义。40%的肺癌患者和57%的抗L-Myc抗体患者检测到抗核抗体。我们的数据表明，抗L-Myc抗体的检测可能有助于诊断和评估一部分肺癌患者对L-Myc的宿主免疫反应。
