Liu H, Ma C, Moses K
Department of Biological Sciences, University of Southern California, Los Angeles 90089-1340, USA.
Mech Dev. 1996 May;56(1-2):73-82. doi: 10.1016/0925-4773(96)00512-6.
The Drosophila melanogaster glass transcription factor acts in photoreceptor cell development, glass transcription in the developing eye begins in the morphogenetic furrow. We report a deletion analysis which shows that a 5147 bp fragment can confer near wild-type expression in the developing eye. We completed the sequence of a similar Drosophila virilis fragment and comparison of these two genomic sequences reveals seven perfectly conserved sequence elements of 15 bp (or longer). We placed oligomers of each element 5' to an hsp70 promoter-lacZ fusion construct, and examined the patterns of beta-galactosidase expression produced in transgenic files. Three of these elements drive beta-galactosidase expression in the developing eye, and one is inhibitory.
果蝇的玻璃转录因子在光感受器细胞发育中起作用，发育中的眼睛中的玻璃转录始于形态发生沟。我们报告了一项缺失分析，该分析表明一个5147 bp的片段可以在发育中的眼睛中赋予接近野生型的表达。我们完成了一个类似的果蝇virilis片段的序列，这两个基因组序列的比较揭示了7个15 bp（或更长）的完全保守的序列元件。我们将每个元件的寡聚物置于hsp70启动子-lacZ融合构建体的5'端，并检查了转基因果蝇中产生的β-半乳糖苷酶表达模式。其中三个元件在发育中的眼睛中驱动β-半乳糖苷酶表达，一个是抑制性的。
