Sokal E M, Baudoux M C, Collette E, Hausleithner V, Lambotte L, Buts J P
Pediatric Liver Research Unit, Faculty of Medicine, Catholic University of Louvain, Brussels, Belgium.
Pediatr Res. 1996 Jul;40(1):66-71. doi: 10.1203/00006450-199607000-00012.
Malnutrition and growth retardation remain a major complication in infants with extrahepatic biliary atresia associated cholestasis. The purpose of this study was to investigate whether oral supplementation with branched chain amino acids (BCAA) can correct malnutrition in a rat model of biliary atresia. Four groups of 15 rats, 30 d old, were used. Group A were shamoperated animals, given a normal laboratory diet (17.5% of caloric intake as proteins). Group B were cholestatic rats (biliary atresia) fed a diet enriched in BCAA (supplement of 8.5%, valine/leucine/isoleucine ratio 1:1:1). Group C were cholestatic mice fed a diet enriched in casein (supplement of 8.5%). Group D were cholestatic mice fed a normal diet. Thirty-two days after surgery, groups were compared for body weight, serum amino acid content, nitrogen balance, muscle mass, and carcass composition. The results showed that the weight of group B, C, and D animals was 85, 81, and 64% of group A (controls). Serum BCAA levels were markedly increased in group B animals. Nitrogen retention was similar in groups B and A, but reduced to 63 and 44% in groups C and D, respectively. Dry weights were similar in group A (39.1% of body weight) and B (37.7%), but reduced to 28.1 and 28.6% of body weight in groups C and D. Body proteins were higher in groups A (13.9%) and B (14.2%) than in group D (9.7%) rats. Mineral content of group B animals was 84% of those of group A, 50% in group C, and 23% in group D rats. It was concluded that an oral supplement of BCAA can correct growth, nitrogen retention, and body composition in experimental biliary atresia. Administration of BCAA supplements to cholestatic infants should be considered.
营养不良和生长发育迟缓仍是肝外胆道闭锁合并胆汁淤积症婴儿的主要并发症。本研究旨在探讨口服补充支链氨基酸（BCAA）是否能纠正胆道闭锁大鼠模型中的营养不良状况。选用4组15只30日龄大鼠。A组为假手术动物，给予正常实验室饮食（热量摄入的17.5%为蛋白质）。B组为胆汁淤积大鼠（胆道闭锁），喂食富含BCAA的饮食（补充8.5%，缬氨酸/亮氨酸/异亮氨酸比例为1:1:1）。C组为胆汁淤积小鼠，喂食富含酪蛋白的饮食（补充8.5%）。D组为胆汁淤积小鼠，喂食正常饮食。术后32天，比较各组的体重、血清氨基酸含量、氮平衡、肌肉质量和胴体组成。结果显示，B、C和D组动物的体重分别为A组（对照组）的85%、81%和64%。B组动物血清BCAA水平显著升高。B组和A组的氮潴留相似，但C组和D组分别降至63%和44%。A组（占体重的39.1%）和B组（占体重的37.7%）的干重相似，但C组和D组分别降至占体重的28.1%和28.6%。A组（13.9%）和B组（14.2%）大鼠的身体蛋白质含量高于D组（9.7%）。B组动物的矿物质含量为A组的84%，C组为50%，D组大鼠为23%。得出的结论是，口服补充BCAA可纠正实验性胆道闭锁中的生长、氮潴留和身体组成。应考虑对胆汁淤积婴儿补充BCAA。
