Bacáková L, Mares V
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Physiol Res. 1995;44(6):389-98.
Migration and proliferation of smooth muscle cells (SMC) were studied in cultures prepared from the aorta of Wistar male rats (170-200 g b.w., 8 weeks old) raised under conventional (CC) or specific pathogen-free (SPF) conditions. In primary cultures, higher movement of cells from explants was found in CC raised donors, namely in samples cultured in serum incomplete medium. In the following subcultures (passage 3-16), the growth curves were steeper and the doubling time shorter in CC type of cultures. The faster growth of SMC population from conventional donors was found to be due to a shorter cell cycle and a higher proportion of dividing cells. As a consequence, the maximum population densities were also higher in the latter type of cultures. The differences in growth, that were dependent on raising conditions, were evident for 16 passages, i.e. 7 months after explantation of cells into culture. The data suggest that breeding conditions may affect the activation of growth of SMC in blood vessels in situ.
在由常规（CC）或无特定病原体（SPF）条件饲养的Wistar雄性大鼠（体重170 - 200克，8周龄）主动脉制备的培养物中，研究了平滑肌细胞（SMC）的迁移和增殖。在原代培养中，在CC饲养的供体中发现来自外植体的细胞迁移更高，即在血清不完全培养基中培养的样本中。在随后的传代培养（第3 - 16代）中，CC型培养物的生长曲线更陡，倍增时间更短。发现来自常规供体的SMC群体生长更快是由于细胞周期更短和分裂细胞比例更高。因此，后一种类型培养物中的最大群体密度也更高。生长差异取决于饲养条件，在传代16次时即细胞接种到培养物中7个月后仍然明显。数据表明饲养条件可能影响原位血管中SMC生长的激活。
