Bacáková L, Mares V, Lisá V, Kocourek F
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
Physiol Res. 1997;46(5):403-6.
The morphology and proliferation of vascular smooth muscle cells (VSMC) were studied in cultures prepared from the aorta of newborn male and female Wistar rats. The doubling times (DT) of the male-derived population were 16.4+/-0.7 h and 30.0+/-2.2 h in the exponential and post-exponential growth phases, respectively. In the female donor cells, the corresponding DT values were significantly longer, i.e. 21.9+/-1.8 h and 38.0+/-2.2 h. In addition, the period of growth was shorter in the female-derived cultures. The percentage of 3H-thymidine labelled cells in male cultures was 61.0+/-3.1, 92.8+/-1.9 and 98.7+/-0.6% at 2, 27 and 52 h, respectively. In the female-derived populations, only 24.6+/-4.4, 66.1+/-3.8 and 82.8+/-2.0% of cells were labelled at the corresponding incubation intervals. As a consequence, the final population density in male cultures was 5.6 times higher. In addition, the male-derived VSMC were mainly spindle-shaped and bulgy in appearance while those from female donors were flat and polygonal which means that the cells were adhering to the growth support to a different extent. The study revealed early determination and long-term persistence of lower adhesiveness as well as higher growth potential of male VSMC, i.e. properties which may be of importance for explaining the higher incidence of vascular wall disorders in males.
对新生雄性和雌性Wistar大鼠主动脉制备的培养物中的血管平滑肌细胞（VSMC）的形态和增殖进行了研究。雄性来源群体在指数生长期和指数生长后期的倍增时间（DT）分别为16.4±0.7小时和30.0±2.2小时。在雌性供体细胞中，相应的DT值明显更长，即21.9±1.8小时和38.0±2.2小时。此外，雌性来源培养物的生长周期较短。雄性培养物中3H-胸腺嘧啶核苷标记细胞的百分比在2、27和52小时分别为61.0±3.1%、92.8±1.9%和98.7±0.6%。在雌性来源群体中，在相应的孵育间隔时，仅24.6±4.4%、66.1±3.8%和82.8±2.0%的细胞被标记。因此，雄性培养物中的最终群体密度高5.6倍。此外，雄性来源的VSMC外观主要呈纺锤形且凸起，而雌性供体的VSMC则扁平且呈多边形，这意味着细胞对生长支持物的粘附程度不同。该研究揭示了雄性VSMC早期确定并长期存在较低粘附性以及较高生长潜力，即这些特性可能对于解释男性血管壁疾病较高的发生率具有重要意义。
