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High-performance liquid chromatographic determination of tramadol in human plasma.

作者信息

Nobilis M, Pastera J, Anzenbacher P, Svoboda D, Kopecký J, Perlík F

机构信息

Institute of Experimental Biopharmaceutics, Joint Research Centre of the Academy of Sciences of the Czech Republic, Hradec Králové, Czech Republic.

出版信息

J Chromatogr B Biomed Appl. 1996 May 31;681(1):177-83. doi: 10.1016/0378-4347(96)88203-x.

DOI:10.1016/0378-4347(96)88203-x
PMID:8798927
Abstract

Tramadol has been determined in human plasma samples using a sensitive high-performance liquid chromatographic method. The plasma samples were extracted with tert.-butylmethyl ether in one-step liquid-liquid extraction (recovery 86%) and analyses of the extracts were performed on reversed-phase silica gel using ion-pair chromatography (verapamil as an internal standard) and fluorescence detection. The method was applied to the determination of tramadol levels in twelve healthy volunteers after oral administration of 100 mg of tramadol in capsules of Protradon and Tramal.

摘要

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