Orci L, Schekman R, Perrelet A
Department of Morphology, University of Geneva Medical Center, Switzerland.
Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8968-70. doi: 10.1073/pnas.93.17.8968.
Intracellular transfers between membrane-bound compartments occur through vesicles that bud from a donor compartment to fuse subsequently with an acceptor membrane. We report that the membrane that delimits COP I or COP II-coated buds/vesicles from the endoplasmic reticulum and the Golgi complex has a thinner interleaflet clear space as compared with the surrounding, noncoated parental membrane. This change is compatible with a compositional change of the membrane bilayer during the budding process.
膜结合区室之间的细胞内转运是通过从供体区室出芽、随后与受体膜融合的囊泡进行的。我们报告称，与周围未被包被的亲代膜相比，界定来自内质网和高尔基体复合体的COP I或COP II包被的芽/囊泡的膜具有更薄的小叶间透明间隙。这种变化与出芽过程中膜双层的成分变化相一致。
