Riley P R, Flint A P, Abayasekara D R, Stewart H J
Institute of Zoology, Regents Park, London, UK.
J Mol Endocrinol. 1995 Oct;15(2):195-202. doi: 10.1677/jme.0.0150195.
A sheep endometrial oxytocin receptor (OTR) cDNA (1.5 kb) was isolated from a lambda-ZAP library using a reverse transcription-PCR product probe generated from oestrous endometrial mRNA. The sheep OTR cDNA shared an overall similarity of 82% with human OTR cDNA, 85% with pig OTR cDNA and 76% with rat OTR cDNA. The encoded receptor was a 391 amino acid polypeptide 94% similar to human OTR, 94% similar to pig OTR and 93% similar to rat OTR. The sheep OTR contained two additional amino acids compared with human OTR which were located in the highly GC-rich third intracytoplasmic loop. This region is thought to be associated with G protein coupling and signal transduction. Expression of the cDNA in Cos-7 cells and measurement of oxytocin-induced phosphoinositide turnover confirmed that it coded for a functional product. The affinity of the expressed receptor was comparable with that observed for the in vivo receptor.
使用从发情期子宫内膜mRNA生成的逆转录-聚合酶链反应（RT-PCR）产物探针，从λ-ZAP文库中分离出绵羊子宫内膜催产素受体（OTR）cDNA（1.5 kb）。绵羊OTR cDNA与人类OTR cDNA的总体相似度为82%，与猪OTR cDNA的相似度为85%，与大鼠OTR cDNA的相似度为76%。编码的受体是一种由391个氨基酸组成的多肽，与人类OTR的相似度为94%，与猪OTR的相似度为94%，与大鼠OTR的相似度为93%。与人类OTR相比，绵羊OTR在富含GC的第三个胞质内环中含有另外两个氨基酸。该区域被认为与G蛋白偶联和信号转导有关。cDNA在Cos-7细胞中的表达以及催产素诱导的磷酸肌醇周转的测量证实它编码一种功能性产物。表达的受体的亲和力与体内受体的亲和力相当。
