Hermant D, Ménard R, Arricau N, Parsot C, Popoff M Y
Unité des Entérobactéries et Unité INSERM 389, Institut Pasteur, Paris, France.
Mol Microbiol. 1995 Aug;17(4):781-9. doi: 10.1111/j.1365-2958.1995.mmi_17040781.x.
A Salmonella typhi chromosomal locus composed of five adjacent genes, designated sipEBCDA, was identified by transposon mutagenesis as being essential for cell invasion. Products of the sip genes exhibit extensive sequence similarities to the effectors of Shigella entry into epithelial cells encoded by the virulence plasmid-borne ipa operon. Expression of sipE and sipB in a Shigella non-invasive ipaB mutant restored the ability to invade epithelial cells. The structural and functional conservation of the Sip and Ipa proteins suggests that Salmonella and Shigella entry processes are promoted by similar effectors.
通过转座子诱变鉴定出一个由五个相邻基因（命名为sipEBCDA）组成的伤寒沙门氏菌染色体位点，该位点对于细胞侵袭至关重要。sip基因的产物与由毒力质粒携带的ipa操纵子编码的志贺氏菌进入上皮细胞的效应子表现出广泛的序列相似性。在志贺氏菌非侵袭性ipaB突变体中表达sipE和sipB可恢复侵袭上皮细胞的能力。Sip和Ipa蛋白的结构和功能保守性表明，沙门氏菌和志贺氏菌的进入过程由相似的效应子促进。
