胃泌素对小鼠腹腔巨噬细胞功能的调节作用

Modulation of murine peritoneal macrophage functions by gastrin.

作者信息

de la Fuente M, Drummond J, del Rio M, Carrasco M, Hernanz A

机构信息

Departamento de Biología Animal II (Fisiología Animal), Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Spain.

出版信息

Peptides. 1996;17(2):219-24. doi: 10.1016/0196-9781(95)02133-7.

DOI:10.1016/0196-9781(95)02133-7

PMID:8801524

Abstract

The effect in vitro of gastrin-17 and gastrin-34 was studied at concentrations from 10(-12) to 10(-6) M on several functions of resting peritoneal macrophages from BALB/c mice: adherence to substrate, mobility (spontaneous and directed by chemical gradient or chemotaxis), and ingestion of inert particles (latex beads) or cells (Candida albicans). Both gastrins, at concentrations from 10(-10) to 10(-8) M, inhibited significantly all functions studied with the exception of adherence, which was increased. A dose-response relationship was observed, with a maximum inhibition of macrophage functions found at 10(-9) M. These peptides induced in murine macrophages a significant increase of cAMP levels at 60 and 120 s. Adenosine, an adenylate cyclase inhibitor, significantly increased the ingestion of latex beads, whereas the combined presence of adenosine and either G-17 or G-34 produced similar values to those of control samples without adenosine or gastrin. These results suggest that gastrin is a negative modulator of several macrophage functions, and that the inhibition of these activities is carried out through an increase of intracellular cAMP levels.

摘要

研究了胃泌素 -17 和胃泌素 -34 在浓度为 10⁻¹² 至 10⁻⁶ M 时对 BALB/c 小鼠静息腹膜巨噬细胞几种功能的体外作用：对底物的黏附、运动性（自发运动以及由化学梯度引导的定向运动或趋化性），以及摄取惰性颗粒（乳胶珠）或细胞（白色念珠菌）。两种胃泌素在浓度为 10⁻¹⁰ 至 10⁻⁸ M 时，除了黏附增加外，显著抑制了所有研究的功能。观察到剂量 - 反应关系，在 10⁻⁹ M 时巨噬细胞功能受到最大抑制。这些肽在 60 秒和 120 秒时使小鼠巨噬细胞中的 cAMP 水平显著升高。腺苷，一种腺苷酸环化酶抑制剂，显著增加了乳胶珠的摄取，而腺苷与 G -17 或 G -34 共同存在时产生的值与不含腺苷或胃泌素的对照样品相似。这些结果表明胃泌素是几种巨噬细胞功能的负调节剂，并且这些活性的抑制是通过细胞内 cAMP 水平的升高来实现的。