Gyöngyössy-Issa M I, Black T, Devine D V
Canadian Red Cross Society Blood Services, University of British Columbia, Vancouver, Canada.
Vox Sang. 1996;70(2):76-85. doi: 10.1111/j.1423-0410.1996.tb01297.x.
To assess alterations in coagulation proteins in stored platelet concentrates, we used a series of platelet parameters and measures of coagulation activation to compare samples collected before unit donation, during the processing of platelet concentrates (PC) from CPDA-1 blood, and in storage up to 5 days as well as stored platelet-poor plasma (PPP). Storage-dependent increases in activated partial thromboplastin time and prothrombin time were seen in both PC and PPP. However, FVII, FXI, FXII, kallikrein activity and prothrombin F1.2 levels remained unchanged in stored PC. Surprisingly, in stored PPP, significant alterations in FXII, FVII, kallikrein and prothrombin F1.2 levels were seen. Platelet morphology and surface marker studies demonstrated platelet activation during storage. These data suggest that the presence of platelets in the CLX storage container partially suppresses coagulation activation at significant cost to platelet viability.
为评估储存血小板浓缩物中凝血蛋白的变化，我们使用了一系列血小板参数和凝血激活指标，比较单位献血前采集的样本、从CPDA-1血液制备血小板浓缩物（PC）过程中的样本、储存长达5天的样本以及储存的少血小板血浆（PPP）。在PC和PPP中均观察到活化部分凝血活酶时间和凝血酶原时间随储存时间增加。然而，储存的PC中FVII、FXI、FXII、激肽释放酶活性和凝血酶原F1.2水平保持不变。令人惊讶的是，在储存的PPP中，FXII、FVII、激肽释放酶和凝血酶原F1.2水平出现了显著变化。血小板形态和表面标志物研究表明储存期间血小板被激活。这些数据表明，CLX储存容器中血小板的存在部分抑制了凝血激活，但以显著降低血小板活力为代价。
