扎伊德拉斯肝癌的线粒体ATP酶。V. 扎伊德拉斯肝癌的线粒体含有与F1不相关的ATP酶复合体膜区。

Mitochondrial ATPase of Zajdela hepatoma. V. Mitochondria of Zajdela hepatoma contain membrane sectors of ATPase complex unassociated with F1.

作者信息

Kuzela S, Kolarov J, Krempaský V, Luciaková K, Ujházy V

出版信息

Neoplasma. 1978;25(6):745-9.

Abstract

Relative content of membrane sectors of ATPase complex in rat liver and Zajdela hepatoma mitochondria and the ability of mitochondrial membrane of the two sources to bind isolated soluble (F1) ATPase were examined. Approximately equal concentrations of oligomycin were required for 50% inhibition of ATPase activity in submitochondrial particles of rat liver and Zajdela hepatoma indicating practically identical content of membrane sectors of ATPase complex in both types of mitochondria. As detected by the increase in oligomycin-sensitive ATPase activity of submitochondrial particles incubated with isolated F1, the submitochondrial particles of Zajdela hepatoma in contrast to those of rat liver were able to bind specifically considerable amounts of exogenously added F1. The results indicate that mitochondria of Zajdela hepatoma contain membrane sectors of ATPase complex unassociated with F1 but capable of association with this enzyme.

摘要

检测了大鼠肝脏和Zajdela肝癌线粒体中ATP酶复合体膜区的相对含量，以及这两种来源的线粒体膜结合分离的可溶性（F1）ATP酶的能力。大鼠肝脏和Zajdela肝癌的亚线粒体颗粒中，50%抑制ATP酶活性所需的寡霉素浓度大致相等，这表明两种类型线粒体中ATP酶复合体膜区的含量实际上相同。通过与分离的F1一起孵育的亚线粒体颗粒中寡霉素敏感性ATP酶活性的增加来检测，与大鼠肝脏的亚线粒体颗粒相比，Zajdela肝癌的亚线粒体颗粒能够特异性结合大量外源添加的F1。结果表明，Zajdela肝癌的线粒体含有与F1不相关但能够与该酶结合的ATP酶复合体膜区。