哺乳动物DNA复制子中重复核苷酸序列的组织方式。
The organization of repeated nucleotide sequences in the replicons of mammalian DNA.
作者信息
Mattern M R, Painter R B
出版信息
Biophys J. 1977 Aug;19(2):117-23. doi: 10.1016/S0006-3495(77)85574-4.
Chinese hamster ovary cells were irradiated with 100-5,000 rads of X-rays and inhibition of the initiation of replicons after irradiation was demonstrated by analyzing nascent DNA sedimented in alkaline sucrose gradients. The renaturation kinetics of DNA synthesized during 60 min of incubation after irradiation was compared with that of DNA synthesized during the 60 min after sham irradiation and with that of parental DNA. Nascent DNA from cells whose replicon initiation was inhibited renatured faster than nascent DNA from control cells in the COt range of repeated nucleotide sequences, suggesting that regions of the replicon not close to origins are enriched in repeated sequences and that regions close to origins are enriched in unique sequences. A class of repeated nucleotide sequences may be involved in the regulation of replicon initiation.
用100 - 5000拉德的X射线照射中国仓鼠卵巢细胞，通过分析碱性蔗糖梯度中沉降的新生DNA，证明了照射后复制子起始的抑制。将照射后孵育60分钟期间合成的DNA的复性动力学与假照射后60分钟期间合成的DNA以及亲本DNA的复性动力学进行了比较。在重复核苷酸序列的Cot范围内，复制子起始受到抑制的细胞中的新生DNA比对照细胞中的新生DNA复性更快，这表明复制子中不靠近起始点的区域富含重复序列，而靠近起始点的区域富含独特序列。一类重复核苷酸序列可能参与复制子起始的调控。
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