Yliheikkilä P K, Masuda T, Ambrose W W, Suggs C A, Felton D A, Cooper L F
Department of Prosthodontics, University of North Carolina, Chapel Hill 27599-7450, USA.
Int J Oral Maxillofac Implants. 1996 Jul-Aug;11(4):456-65.
Bone formation at implant surfaces may be directly influenced by effects of the implant material on osteoblast behavior. Cell culture models of osteoblast physiology may be used to investigate the interaction of osteoblastic cells with various surfaces. In this study, primary cultured fetal bovine mandibular osteoblastic cells were cultured on titanium, ceramic hydroxyapatite, and glass coverslip surfaces to allow for the comparison of the mineralizing matrix elaborated by osteoblasts grown on different implant material surfaces. Morphologic and immunohistochemical analysis revealed the similar formation of multilayered, mineralizing cultures on these three surfaces. The qualitative similarity of the matrix formed on these culture surfaces may reflect similar qualitative in vivo responses of bone to titanium and hydroxyapatite implants.
种植体表面的骨形成可能直接受到种植体材料对成骨细胞行为影响的作用。成骨细胞生理学的细胞培养模型可用于研究成骨细胞与各种表面的相互作用。在本研究中，将原代培养的胎牛下颌骨成骨细胞培养在钛、陶瓷羟基磷灰石和玻璃盖玻片表面，以便比较在不同种植体材料表面生长的成骨细胞所形成的矿化基质。形态学和免疫组织化学分析显示，在这三种表面上形成了类似的多层矿化培养物。在这些培养表面形成的基质在质量上的相似性可能反映了骨在体内对钛和羟基磷灰石种植体的类似质量反应。
