Cooper L F, Masuda T, Whitson S W, Yliheikkilä P, Felton D A
Department of Prosthodontics, University of North Carolina School of Dentistry, Chapel Hill, USA.
Int J Oral Maxillofac Implants. 1999 Jan-Feb;14(1):37-47.
Altering osseous responses at implant surfaces to enhance bone is a current goal of clinical therapy. Cell culture may be used to investigate surface-dependent responses of bone-forming cells. In this report, the ability of primary fetal bovine mandibular osteoblast cultures to form a mineralizing matrix on machined, titanium plasma-sprayed, and titanium oxide grit-blasted surfaces has been compared. Immunohistochemical markers associated with bone formation were used to define the differentiated state of the formed matrix using qualitative light microscopy, and von Kossa staining was used to demonstrate the presence of mineralization within this matrix. Compared to either titanium oxide grit-blasted or machined surfaces, titanium plasma-sprayed surfaces displayed a unique pattern of mineralized matrix formation. Scanning electron microscopy further revealed that each surface accumulated unique organic and inorganic deposits during matrix formation, suggesting that surface-dependent physicochemical and biochemical conditioning of implant surfaces takes place. Surface topographic features of commercially pure titanium substrates can alter cultured osteoblast extracellular matrix formation and mineralization. Similar molecular and cellular assessment of in vivo responses to implant surface topography may contribute to improved engineering of endosseous implants.
改变种植体表面的骨反应以促进骨生长是当前临床治疗的目标。细胞培养可用于研究成骨细胞的表面依赖性反应。在本报告中，比较了原代胎牛下颌骨成骨细胞培养物在机械加工、钛等离子喷涂和氧化钛喷砂处理表面上形成矿化基质的能力。使用与骨形成相关的免疫组织化学标记物，通过定性光学显微镜确定所形成基质的分化状态，并使用冯·科萨染色法证明该基质中矿化的存在。与氧化钛喷砂处理或机械加工表面相比，钛等离子喷涂表面呈现出独特的矿化基质形成模式。扫描电子显微镜进一步显示，每个表面在基质形成过程中积累了独特的有机和无机沉积物，这表明种植体表面发生了表面依赖性的物理化学和生物化学调节。商业纯钛基底的表面形貌特征可改变培养的成骨细胞细胞外基质的形成和矿化。对植入物表面形貌的体内反应进行类似的分子和细胞评估可能有助于改进骨内植入物的工程设计。
