Soffredini R, Rumi M, Lampertico P, Aroldi A, Tarantino A, Ponticelli C, Colombo M
Institute of Internal Medicine, IRCCS Ospedale Maggiore, University of Milan, Italy.
Am J Kidney Dis. 1996 Sep;28(3):437-40. doi: 10.1016/s0272-6386(96)90503-x.
To assess the sensitivity and specificity of third-generation assays for antibody to hepatitis C virus (anti-HCV), sera from 244 renal transplant patients (113 positive for anti-HCV enzyme-linked immunosorbent assay [ELISA]-2) were studied. Hepatitis C virus RNA was detected by a reverse-transcripted nested polymerase chain reaction. Antibody to HCV was detected by ELISA-3 in 108 (96%) ELISA-2-positive samples. Five (4%) ELISA-2-positive sera were negative by both ELISA-3 and polymerase chain reaction. In the anti-HCV-negative group, six (5%) additional cases were ELISA-3-positive; three of these were confirmed by recombinant immunoblot assay-3 (RIBA-3) and polymerase chain reaction. Recombinant immunoblot assay-3 was used to resolve 82 RIBA-2-indeterminate and three RIBA-2-negative sera. Using RIBA-3, 49 (60%) RIBA-2-indeterminate samples were positive, five (6%) ELISA-3-negative samples were negative, and 28 (34%) were remained indeterminate. Recombinant immunoblot assay-2-negative samples were indeterminate with RIBA-3. Hepatitis C virus RNA was detected in all RIBA-3-positive and 58% of the RIBA-3-indeterminate samples. Third-generation assays for anti-HCV are more sensitive and specific than second-generation assays in renal transplant patients.
为评估丙型肝炎病毒抗体（抗-HCV）第三代检测法的敏感性和特异性，对244例肾移植患者（113例抗-HCV酶联免疫吸附测定[ELISA]-2呈阳性）的血清进行了研究。采用逆转录巢式聚合酶链反应检测丙型肝炎病毒RNA。在108份（96%）ELISA-2阳性样本中，ELISA-3检测到抗-HCV。5份（4%）ELISA-2阳性血清经ELISA-3和聚合酶链反应检测均为阴性。在抗-HCV阴性组中，另有6例（5%）ELISA-3呈阳性；其中3例经重组免疫印迹法-3（RIBA-3）和聚合酶链反应确认。使用重组免疫印迹法-3对82份RIBA-2结果不确定和3份RIBA-2阴性的血清进行分析。采用RIBA-3时，49份（60%）RIBA-2结果不确定的样本呈阳性，5份（6%）ELISA-3阴性样本呈阴性，28份（34%）仍结果不确定。RIBA-2阴性样本经RIBA-3检测结果仍不确定。在所有RIBA-3阳性样本和58%的RIBA-3结果不确定样本中检测到丙型肝炎病毒RNA。在肾移植患者中，抗-HCV第三代检测法比第二代检测法更敏感、更特异。
