Gabrielian A, Simoncsits A, Pongor S
International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
FEBS Lett. 1996 Sep 9;393(1):124-30. doi: 10.1016/0014-5793(96)00837-x.
Local bending propensity and curvature of DNA can be characterized using a vector description of DNA bendability, based on a set of parameters derived from deoxyribonuclease I (DNase I) cleavage experiments. Two characteristics-arithmetic and vector averages of bendability-were successfully used to predict experimentally known bendable, rigid and curved segments in DNA. A characteristic distribution of bendability is conserved in evolutionarily related kinetoplast sequences. An analysis of the M. genitalium and H. influenzae genomes as well as fragments of human and yeast genomes shows, on the other hand, that highly curved segments--similar to artificially designed curved oligonucleotides--are extremely rare in natural DNA.
基于从脱氧核糖核酸酶I（DNase I）切割实验中获得的一组参数，DNA的局部弯曲倾向和曲率可以通过DNA弯曲能力的矢量描述来表征。弯曲能力的两个特征——算术平均值和矢量平均值——成功地用于预测实验中已知的DNA中可弯曲、刚性和弯曲的片段。弯曲能力的特征分布在进化相关的动质体序列中是保守的。另一方面，对生殖支原体和流感嗜血杆菌基因组以及人类和酵母基因组片段的分析表明，在天然DNA中，高度弯曲的片段——类似于人工设计的弯曲寡核苷酸——极其罕见。
