Palli S R, Caputo G F, Sohi S S, Brownwright A J, Ladd T R, Cook B J, Primavera M, Arif B M, Retnakaran A
Natural Resources Canada, Canadian Forest Service, Sault Ste. Marie, Ontario, Canada.
Virology. 1996 Aug 1;222(1):201-13. doi: 10.1006/viro.1996.0411.
Morphological and molecular changes produced by Autographa californica nuclear polyhedrosis virus (AcMNPV) infection in a permissive cell line, IPLB-SF-21AE (SF-21), of Spodoptera frugiperda and a nonpermissive cell line, FPMI-CF-203 (CF-203), of Choristoneura fumiferana are described. CF-203 cells inoculated with AcMNPV showed a DNA ladder and morphological changes such as plasma membrane granulation, blebbing, and nuclear fragmentation, which are characteristic of apoptosis. Typical virus replication and occlusion body (OB) production were seen in SF-21 cells inoculated with AcMNPV and no apoptosis-like symptoms were observed. mRNA for the apoptosis suppressor gene p35 was detected 9 hr later in AcMNPV-inoculated CF-203 cells than in SF-21 cells. Only a trace amount of mRNA for the AcMNPV-inhibitor of apoptosis homologue (Ac-iap) gene and no mRNAs for the late genes, AcMNPV-polyhedrin (Ac-polh) and AcMNPV-p10 (Ac-p10), were detected in AcMNPV-inoculated CF-203 cells. Inoculation of CF-203 cells with CfMNPV at least 12 hr prior to inoculation with AcMNPV prevented apoptosis-like cell death, and mRNAs for Ac-iap, Ac-polh, and Ac-p10 genes were expressed resulting in successful virus replication and OB production.
本文描述了苜蓿银纹夜蛾核型多角体病毒（AcMNPV）感染草地贪夜蛾的敏感细胞系IPLB - SF - 21AE（SF - 21）以及云杉芽卷叶蛾的非敏感细胞系FPMI - CF - 203（CF - 203）后所产生的形态学和分子变化。接种AcMNPV的CF - 203细胞出现了DNA梯状条带以及诸如质膜颗粒化、起泡和核碎片化等形态学变化，这些都是凋亡的特征。在接种AcMNPV的SF - 21细胞中观察到了典型的病毒复制和包涵体（OB）产生，且未观察到类似凋亡的症状。接种AcMNPV后9小时，在CF - 203细胞中检测到凋亡抑制基因p35的mRNA，而在SF - 21细胞中未检测到。在接种AcMNPV的CF - 203细胞中，仅检测到微量的AcMNPV凋亡抑制同源物（Ac - iap）基因的mRNA，未检测到晚期基因AcMNPV - 多角体蛋白（Ac - polh）和AcMNPV - p10（Ac - p10）的mRNA。在接种AcMNPV前至少12小时用CfMNPV接种CF - 203细胞可防止类似凋亡的细胞死亡，并且Ac - iap、Ac - polh和Ac - p10基因的mRNA得以表达，从而成功实现病毒复制和OB产生。
