用于检测人型支原体的诊断性聚合酶链反应检测方法的开发。

Development of a diagnostic polymerase chain reaction assay for detection of Mycoplasma hominis.

作者信息

Gallia G L, Petroziello J M, Brogan J M, McCleskey F K, DelVecchio V G

机构信息

Molecular Biology Institute, University of Scranton, PA 18510-4625, USA.

出版信息

Mol Cell Probes. 1995 Dec;9(6):415-21. doi: 10.1006/mcpr.1995.0064.

DOI:10.1006/mcpr.1995.0064

PMID:8808312

Abstract

A polymerase chain reaction (PCR)-based assay was developed for the detection of Mycoplasma hominis. This assay generates a 152-bp PCR product which was part of an initial 471-bp M. hominis genomic DNA fragment. The 471-bp DNA fragment was shown by hybridization analysis to be unique for M. hominis. The PCR assay can amplify as few as 18 molecules of target DNA. This diagnostic assay offers potential for wide clinical application as it is rapid and can be successfully performed on crude sample preparations from a variety of media or biopsies. The use of this assay should aid in defining the aetiologic and pathologic roles played by M. hominis and thereby benefit patients.

摘要

开发了一种基于聚合酶链反应（PCR）的检测人型支原体的方法。该方法产生一个152 bp的PCR产物，它是最初471 bp人型支原体基因组DNA片段的一部分。通过杂交分析表明，471 bp的DNA片段是人型支原体特有的。该PCR检测方法能够扩增低至18个分子的靶DNA。这种诊断方法具有广泛的临床应用潜力，因为它速度快，并且可以成功地对来自各种培养基或活检组织的粗样品制备物进行检测。使用该检测方法应有助于明确人型支原体所起的病因学和病理学作用，从而使患者受益。