Ohta K, Nakamura M, Hirokawa K, Tanaka S, Iwama A, Suda T, Ando M, Tanaka H
Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, Japan.
Mech Dev. 1996 Jan;54(1):59-69. doi: 10.1016/0925-4773(95)00461-0.
Receptor tyrosine kinases (RTKs) play important roles in cellular proliferation, differentiation, and survival. We performed reverse transcriptase-polymerase chain reactions (RT-PCR) from enriched embryonic day 5 (E5) chick motoneurons by panning to identify RTKs involved in the early development of motoneuron. In situ hybridization revealed that Cek8, a member of the eph family, was specifically expressed on motoneurons at the brachial and lumbar segments of the spinal cord which innervate limb muscles, and disappeared after the naturally occurring cell death period (E6-E11). Immunohistochemistry using an anti-Cek8 monoclonal antibody showed the localization of Cek8 protein at the cell bodies and axonal fibers of motoneurons and muscles. The unique expression of Cek8 suggests its involvement in cellular survival or cell-cell interactions for specific subpopulations of developing motoneurons.
受体酪氨酸激酶（RTKs）在细胞增殖、分化和存活中发挥着重要作用。我们通过淘选从富集的胚胎第5天（E5）鸡运动神经元中进行逆转录聚合酶链反应（RT-PCR），以鉴定参与运动神经元早期发育的RTKs。原位杂交显示，eph家族成员Cek8在脊髓臂段和腰段支配肢体肌肉的运动神经元上特异性表达，并在自然发生的细胞死亡期（E6-E11）后消失。使用抗Cek8单克隆抗体的免疫组织化学显示Cek8蛋白定位于运动神经元和肌肉的细胞体及轴突纤维。Cek8的独特表达表明它参与了发育中运动神经元特定亚群的细胞存活或细胞间相互作用。
