铜绿假单胞菌的uvrB基因不是DNA损伤诱导型的。
The uvrB gene of Pseudomonas aeruginosa is not DNA damage inducible.
作者信息
Rivera E, Vila L, Barbé J
机构信息
Department of Genetics and Microbiology, Autonomous University of Barcelona Bellaterra, Spain.
出版信息
J Bacteriol. 1996 Sep;178(18):5550-4. doi: 10.1128/jb.178.18.5550-5554.1996.
Abstract
The uvrB gene of Pseudomonas aeruginosa has been isolated from a genomic library by complementation of an Escherichia coli uvrB mutant. The complete nucleotide sequence of P. aeruginosa uvrB consists of 2,013 bp, encoding a polypeptide of 670 amino acids. A P. aeruginosa SOS consensus region, which functions as a binding site for the LexA repressor molecule, is not present in the upstream region of the uvrB gene isolated. By transcriptional fusions with a reporter gene, it has been demonstrated that, contrary to what happens with the homologous gene of E. coli, the P. aeruginosa uvrB gene is not DNA damage inducible. Nevertheless, the UvrB protein must be functional in P. aeruginosa cells because a uvrB-defective mutant is extremely sensitive to UV radiation.
摘要
通过对大肠杆菌uvrB突变体进行互补，从基因组文库中分离出了铜绿假单胞菌的uvrB基因。铜绿假单胞菌uvrB的完整核苷酸序列由2013个碱基对组成，编码一个含有670个氨基酸的多肽。在所分离的uvrB基因的上游区域不存在作为LexA阻遏分子结合位点的铜绿假单胞菌SOS共有区域。通过与报告基因进行转录融合，已证明与大肠杆菌的同源基因不同，铜绿假单胞菌uvrB基因不是DNA损伤诱导型的。然而，UvrB蛋白在铜绿假单胞菌细胞中一定是有功能的，因为uvrB缺陷型突变体对紫外线辐射极其敏感。
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